细胞壁锚定的糖蛋白赋予口放线菌生物膜对阳离子应激的抗性。

IF 2.8 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Molecular Oral Microbiology Pub Date : 2022-10-01 Epub Date: 2022-03-25 DOI:10.1111/omi.12365
Abu Amar M Al Mamun, Chenggang Wu, Chungyu Chang, Belkys C Sanchez, Asis Das, Hung Ton-That
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引用次数: 1

摘要

口腔放线菌在口腔生物膜的发育中起着重要作用。像许多革兰氏阳性细菌一样,A.oris产生大量的表面蛋白,这些蛋白通过一种名为家政分拣酶SrtA的保守转肽酶锚定在细菌肽聚糖上;然而,许多口腔A.oris表面蛋白在生物膜形成中的生物学作用在很大程度上是未知的。在这里,我们报道了糖蛋白GspA-srtA缺失致死性的遗传抑制剂,它不仅促进生物膜的形成,而且在阳离子胁迫下保持细胞膜的完整性。与野生型细胞相比,在单价和二价阳离子浓度升高的情况下,缺乏gspA的突变细胞形成的单物种和多物种生物膜显著减少,尽管在阳离子存在的情况下这两种细胞类型的浮游生长难以区分。由于gspA过表达对缺乏gspA和srtA的细胞是致命的,我们进行了基因筛选,以确定涉及细胞活力的gspA决定因素。活克隆的DNA测序和生物化学特征表明,两个关键半胱氨酸残基和一个丝氨酸残基的突变严重影响GspA的糖基化和生物膜的形成。此外,缺乏gspA的突变细胞对十二烷基硫酸钠(一种溶解质膜的洗涤剂)显著敏感,这表明gspA突变株的细胞包膜发生了改变。与这一观察结果一致,与野生型菌株相比,gspA突变体表现出增加的膜渗透性,与gspA糖基化无关。总之,这些结果支持这样一种观点,即细胞壁锚定的糖蛋白GspA在a.oris促进的生物膜发育中提供了对抗阳离子应激的防御机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A cell wall-anchored glycoprotein confers resistance to cation stress in Actinomyces oris biofilms.

Actinomyces oris plays an important role in oral biofilm development. Like many gram-positive bacteria, A. oris produces a sizable number of surface proteins that are anchored to bacterial peptidoglycan by a conserved transpeptidase named the housekeeping sortase SrtA; however, the biological role of many A. oris surface proteins in biofilm formation is largely unknown. Here, we report that the glycoprotein GspA-a genetic suppressor of srtA deletion lethality-not only promotes biofilm formation but also maintains cell membrane integrity under cation stress. In comparison to wild-type cells, under elevated concentrations of mono- and divalent cations the formation of mono- and multi-species biofilms by mutant cells devoid of gspA was significantly diminished, although planktonic growth of both cell types in the presence of cations was indistinguishable. Because gspA overexpression is lethal to cells lacking gspA and srtA, we performed a genetic screen to identify GspA determinants involving cell viability. DNA sequencing and biochemical characterizations of viable clones revealed that mutations of two critical cysteine residues and a serine residue severely affected GspA glycosylation and biofilm formation. Furthermore, mutant cells lacking gspA were markedly sensitive to sodium dodecyl sulfate, a detergent that solubilizes the cytoplasmic membranes, suggesting the cell envelope of the gspA mutant was altered. Consistent with this observation, the gspA mutant exhibited increased membrane permeability, independent of GspA glycosylation, compared to the wild-type strain. Altogether, the results support the notion that the cell wall-anchored glycoprotein GspA provides a defense mechanism against cation stress in biofilm development promoted by A. oris.

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来源期刊
Molecular Oral Microbiology
Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY
CiteScore
6.50
自引率
5.40%
发文量
46
审稿时长
>12 weeks
期刊介绍: Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.
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