Lncrna CASC11通过靶向FoxO1加重糖尿病肾病。

IF 2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Medical Biochemistry Pub Date : 2023-08-25 DOI:10.5937/jomb0-42345

Yun Zhang, Shuhan Shi, Changda Lin, Lishuang Che, Yuangen Li, Quanzuan Zeng, Weiyuan Lin

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引用次数: 0

摘要

背景：探讨CASC11通过调节FoxO1（forkhead transcription factor O1）对加重型糖尿病肾病（DN）的生物学作用。方法：检测DN患者血清CASC11和FoxO1水平。通过绘制ROC曲线分析CASC11预测DN发病的可能性。采用Pearson相关检验评价CASC11与FoxO1的相关性。在干预CASC11和FoxO1水平后，我们发现高糖（HG）诱导的肾系膜细胞的增殖和迁移能力分别发生了变化。通过蛋白质印迹检测CASC11和FoxO1调节的TGF-β1和Smads的蛋白质水平。

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Lncrna CASC11 aggravates diabetic nephropathy via targeting FoxO1.

Background: To explore the biological effects of CASC11 on aggravating diabetic nephropathy (DN) by regulating FoxO1 (forkhead transcription factor O1).

Methods: Serum levels of CASC11 and FoxO1 in DN patients were detected. The possibility of CASC11 in predicting the onset of DN was analyzed by depicting ROC curves. Correlation between CASC11 and FoxO1 was evaluated by Pearson correlation test. After intervening CASC11 and FoxO1 levels, we found that changes in proliferative and migratory abilities in high glucose (HG)induced kidney mesangial cells were determined respectively. Protein levels of TGF-β1 and Smads regulated by both CASC11 and FoxO1 were examined by Western blot.

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来源期刊

Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

12.00%

发文量

审稿时长

>12 weeks

期刊介绍： The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.