【TGFβ1抗体在IHC损伤活力测定中的应用。实验结果】。

Q4 Medicine

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2023-01-01 DOI:10.17116/sudmed20236605143

M V Fedulova, D D Kupriyanov

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引用次数: 0

摘要

本研究的目的：检验TGFβ1抗体IHC染色法在损伤活力测定中的适用性。来自12人的72份机械损伤皮肤尸检样本被分为3组（每组n=24）：生命损伤、死后损伤和对照非损伤样本。使用标准H&E染色和带有TGFβ1抗体的IHC进行常规组织学检查。我们得出结论，TGFβ1染色在严重损伤皮肤的角质形成细胞中呈阳性，在死后损伤和对照非损伤样本中呈阴性。讨论了TGFβ1在损伤愈合过程中的作用，以及TGFβ。

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[TGFβ1 antibodies application for IHC determination of injuries vitality. The experimental results].

The aim of the study: Is to examine the applicability of IHC staining method with TGFβ1 antibodies for injuries vitality determination. 72 skin autopsy samples with mechanical injuries from 12 persons were divided to 3 groups (n=24 in each group): vital injuries, postmortal injuries, control non-injured samples. A routine histological examination using standard H&E stain and IHC with TGFβ1 antibodies was performed. We concluded the positive TGFβ1 staining was in keratinocytes in vitally injured skin and the negative one in postmortally injured and control non-injured samples. The role of TGFβ1 in the injuries healing process and the further examinations of the TGFβ1 staining method application for determination of injuries vitality are discussed.

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来源期刊

Sudebno-Meditsinskaya Ekspertisa Medicine-Pathology and Forensic Medicine

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The journal is concerned with the theory and practice of forensic medicine - the problems of thanatology, traumatology, toxicology, serology, forensic obstetrics, forensic dentistry, forensic psychiatry, forensic chemistry, physicotechnical methods of investigation, history of forensic medicine and some problems of criminology and legal laws related to forensic medicine. It publishes original studies by Russian authors, casuistry surveys, abstracts and reviews of Russian and foreign literature, scientific information, reports on scientific conferences.