Amanda Goulart , Naira Ferreira Anchieta , Pedro Alexandre Sampaio , Vânia Brazão , Jefferson Luiz Da Silva , Gisele Bulhões Portapilla , Andressa Duarte , Daiane Yukie Tezuca , Maiara Voltarelli Providello , Angelita Maria Stabile , José Clóvis do Prado Júnior
{"title":"自助餐厅饮食诱导的肥胖重塑了急性克氏锥虫感染的免疫反应。","authors":"Amanda Goulart , Naira Ferreira Anchieta , Pedro Alexandre Sampaio , Vânia Brazão , Jefferson Luiz Da Silva , Gisele Bulhões Portapilla , Andressa Duarte , Daiane Yukie Tezuca , Maiara Voltarelli Providello , Angelita Maria Stabile , José Clóvis do Prado Júnior","doi":"10.1016/j.imbio.2023.152747","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Obesity is a global problem associated with several conditions, including hypertension, diabetes, arthritis and cardiovascular diseases. With the increase in the prevalence of obesity in recent years, mostly in developing countries, it is important to study its impact on various diseases, including infectious illnesses, such as Chagas disease, caused by the protozoan <em>Trypanosoma cruzi</em>. Considering that a diet rich in salt, sugar, and fat is associated with obesity, this study aimed to evaluate the influence of cafeteria diet (CAF)-induced obesity on immune responses in <em>T. cruzi</em>-infected rats.</p></div><div><h3>Methods</h3><p>Male <em>Wistar</em> Hannover rats were provided with water and food <em>ad libitum</em> (chow group). The CAF-fed groups received a normal rodent diet or CAF. The animals were intraperitoneally infected with 10<sup>5</sup> trypomastigote forms of the Y strain of <em>T. cruzi</em> present in the whole blood from a previously infected mouse.</p></div><div><h3>Results</h3><p>CAF-fed rats showed a significant increase in visceral adipose tissue weight compared to chow-fed rats. A significant reduction in CD3<sup>+</sup> CD4<sup>+</sup> helper splenic T cells was observed in obese-infected rats compared to non-obese-infected rats, as well as CD11b and macrophages. In addition, macrophages from obese animals displayed reduced RT1b levels compared to those from control animals. Moreover, INF-γ, an important factor in macrophage activation, was reduced in obese-infected rats compared with their counterparts.</p></div><div><h3>Conclusions</h3><p>These results indicate that a CAF can impair the cell-mediated immune response against <em>T. cruzi.</em></p></div>","PeriodicalId":13270,"journal":{"name":"Immunobiology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cafeteria diet-induced obesity remodels immune response in acute Trypanosoma cruzi infection\",\"authors\":\"Amanda Goulart , Naira Ferreira Anchieta , Pedro Alexandre Sampaio , Vânia Brazão , Jefferson Luiz Da Silva , Gisele Bulhões Portapilla , Andressa Duarte , Daiane Yukie Tezuca , Maiara Voltarelli Providello , Angelita Maria Stabile , José Clóvis do Prado Júnior\",\"doi\":\"10.1016/j.imbio.2023.152747\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Obesity is a global problem associated with several conditions, including hypertension, diabetes, arthritis and cardiovascular diseases. With the increase in the prevalence of obesity in recent years, mostly in developing countries, it is important to study its impact on various diseases, including infectious illnesses, such as Chagas disease, caused by the protozoan <em>Trypanosoma cruzi</em>. Considering that a diet rich in salt, sugar, and fat is associated with obesity, this study aimed to evaluate the influence of cafeteria diet (CAF)-induced obesity on immune responses in <em>T. cruzi</em>-infected rats.</p></div><div><h3>Methods</h3><p>Male <em>Wistar</em> Hannover rats were provided with water and food <em>ad libitum</em> (chow group). The CAF-fed groups received a normal rodent diet or CAF. The animals were intraperitoneally infected with 10<sup>5</sup> trypomastigote forms of the Y strain of <em>T. cruzi</em> present in the whole blood from a previously infected mouse.</p></div><div><h3>Results</h3><p>CAF-fed rats showed a significant increase in visceral adipose tissue weight compared to chow-fed rats. A significant reduction in CD3<sup>+</sup> CD4<sup>+</sup> helper splenic T cells was observed in obese-infected rats compared to non-obese-infected rats, as well as CD11b and macrophages. In addition, macrophages from obese animals displayed reduced RT1b levels compared to those from control animals. Moreover, INF-γ, an important factor in macrophage activation, was reduced in obese-infected rats compared with their counterparts.</p></div><div><h3>Conclusions</h3><p>These results indicate that a CAF can impair the cell-mediated immune response against <em>T. cruzi.</em></p></div>\",\"PeriodicalId\":13270,\"journal\":{\"name\":\"Immunobiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2023-09-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunobiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0171298523045497\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunobiology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0171298523045497","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Obesity is a global problem associated with several conditions, including hypertension, diabetes, arthritis and cardiovascular diseases. With the increase in the prevalence of obesity in recent years, mostly in developing countries, it is important to study its impact on various diseases, including infectious illnesses, such as Chagas disease, caused by the protozoan Trypanosoma cruzi. Considering that a diet rich in salt, sugar, and fat is associated with obesity, this study aimed to evaluate the influence of cafeteria diet (CAF)-induced obesity on immune responses in T. cruzi-infected rats.
Methods
Male Wistar Hannover rats were provided with water and food ad libitum (chow group). The CAF-fed groups received a normal rodent diet or CAF. The animals were intraperitoneally infected with 105 trypomastigote forms of the Y strain of T. cruzi present in the whole blood from a previously infected mouse.
Results
CAF-fed rats showed a significant increase in visceral adipose tissue weight compared to chow-fed rats. A significant reduction in CD3+ CD4+ helper splenic T cells was observed in obese-infected rats compared to non-obese-infected rats, as well as CD11b and macrophages. In addition, macrophages from obese animals displayed reduced RT1b levels compared to those from control animals. Moreover, INF-γ, an important factor in macrophage activation, was reduced in obese-infected rats compared with their counterparts.
Conclusions
These results indicate that a CAF can impair the cell-mediated immune response against T. cruzi.
期刊介绍：
Immunobiology is a peer-reviewed journal that publishes highly innovative research approaches for a wide range of immunological subjects, including
• Innate Immunity,
• Adaptive Immunity,
• Complement Biology,
• Macrophage and Dendritic Cell Biology,
• Parasite Immunology,
• Tumour Immunology,
• Clinical Immunology,
• Immunogenetics,
• Immunotherapy and
• Immunopathology of infectious, allergic and autoimmune disease.
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