多西他赛对小鼠II期卵母细胞玻璃化过程中自噬相关基因的保护作用。

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL
Hamed Daneshpazhouh, Nasim Hayati Roodbari, Yaser Tahamtani, Zahra Khodabandeh, Mehdi Dianatpour
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引用次数: 0

摘要

背景:自噬是细胞生存的一种保守机制,是细胞对应激条件的主要反应。本研究旨在评估多西他赛对玻璃化卵母细胞存活、受精和自噬相关基因表达的影响。方法:该研究于2018年在伊朗设拉子医学科学大学干细胞技术研究中心进行。将变性的卵母细胞随机选择并分为五组,即对照组(n=133)、多西他赛组(n=136)、多西他赛+冷冻保护剂组(n=146)、多西他赛+玻璃化处理组(n=138)和玻璃化处理(n=145)。玻璃化处理对自噬相关基因5(ATG5)和Beclin-1表达的影响通过实时聚合酶链反应测定。使用SPSS软件(版本26.0)和GraphPad Prism 9对数据进行分析。结果:与对照组相比,各实验组的存活率和受精率均显著降低(P=0.001)。卵母细胞体外受精后,多西他赛+玻璃化和玻璃化组中的2-细胞形成率与对照组和多西他塞尔组相比显著降低(分别为P=0.001和P=0.001)。在多西他赛+冷冻保护剂和多西他塞尔+玻璃化组中,用多西他塞预孵育卵母细胞降低了Beclin-1和ATG5的基因表达水平(分别为P=0.001和P=0.019)。与对照组相比,多西他赛组的这些基因表达水平也降低了(P=0.001)。结论:与未处理的卵母细胞相比,玻璃化前用多西他塞尔孵育小鼠中期II卵母细胞降低了自噬相关基因的表达,并提高了存活率和受精率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes.

Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes.

Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes.

Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes.

Background: Autophagy is a conservative mechanism for cell survival as the main response of cells to stress conditions. The present study aimed to assess the effect of docetaxel on the survival, fertilization, and expression of autophagy-related genes in vitrified oocytes.

Methods: The study was conducted in 2018 at the Stem Cells Technology Research Center, Shiraz University of Medical Sciences (Shiraz, Iran). Denuded oocytes were randomly selected and assigned to five groups, namely control (n=133), docetaxel (n=136), docetaxel+cryoprotectants (n=146), docetaxel+vitrification (n=138), and vitrification (n=145). The effect of vitrification on the expression of autophagy-related gene 5 (ATG5) and Beclin-1 was determined using a real-time polymerase chain reaction. Data were analyzed using SPSS software (version 26.0) and GraphPad Prism 9.

Results: Survival and fertilization rates in each experimental group were significantly reduced compared to the control group (P=0.001). After in vitro fertilization of oocytes, the 2-cell formation rate was significantly reduced in the docetaxel+vitrification and vitrification groups compared to the control and docetaxel groups (P=0.001 and P=0.001, respectively). Pre-incubation of oocytes with docetaxel reduced gene expression levels of Beclin-1 and ATG5 in the docetaxel+cryoprotectants and docetaxel+vitrification groups (P=0.001 and P=0.019, respectively). The expression level of these genes was also reduced in the docetaxel group compared to the control group (P=0.001).

Conclusion: Incubation of mouse metaphase II oocytes with docetaxel prior to vitrification reduced the expression of autophagy-related genes and increased survival and fertilization rates compared to untreated oocytes.

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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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