用表达基于荧光泛素化的细胞周期指标的人宫颈癌HeLa细胞进行有丝分裂收获方法的验证,用于辐射研究。

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Ryosuke Seino, Hiroto Uno, Hisanori Fukunaga
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引用次数: 0

摘要

细胞周期是一个细胞产生两个子细胞过程中的一系列事件。Terasima和Tolmach在20世纪60年代建立的有丝分裂收获方法,通过仅从培养的细胞系统中收集有丝分裂细胞,对细胞造成最小的生理压力,并实现高度的细胞周期同步。本研究的目的是验证使用表达基于荧光泛素化的细胞周期指标(FUCCI)的人宫颈细胞系HeLa细胞的有丝分裂收获方法的多功能性,并估计HeLa FUCCI细胞中放射敏感性的细胞周期依赖性变化。图像分析显示,在有丝分裂细胞收集后,细胞周期同步性保持至少24小时。此外,克隆形成试验显示放射敏感性的变化是细胞周期依赖性的。这些结果表明,使用表达FUCCI的细胞的有丝分裂收获方法在辐射细胞生物学领域具有高度的通用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Validation of mitotic harvesting method with human cervical carcinoma HeLa cells expressing fluorescent ubiquitination-based cell cycle indicators for radiation research.
The cell cycle is a series of events in the process of one cell giving rise to two daughter cells. The mitotic harvesting method, established by Terasima and Tolmach in the 1960s, causes minimal physiological stress on the cells and achieves a high degree of cell cycle synchrony by collecting only mitotic cells from a cultured cell system. The purpose of the present study is to validate the versatility of the mitotic harvesting method using human cervical cell line HeLa cells expressing Fluorescent Ubiquitination-based Cell Cycle Indicators (FUCCI) and to estimate the cell cycle-dependent changes in radiosensitivity in HeLa-FUCCI cells. The image analysis showed that cell cycle synchrony was maintained for at least 24 hours after mitotic cell collection. Also, the clonogenic assay demonstrated changes in radiosensitivity that were cell cycle dependent. These results indicate that the mitotic harvesting method using FUCCI-expressing cells has high versatility in the field of radiation cell biology.
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来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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