电刺激对后肢空载大鼠比目鱼肌蛋白质合成和分解的急性影响。

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Miho Kanazashi, Masayuki Tanaka
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引用次数: 0

摘要

电刺激(ES)对废用性肌肉萎缩有效。然而,ES对肌肉蛋白合成(MPS)和肌肉蛋白分解(MPB)的急性影响尚不清楚。我们研究了单次ES治疗对2周后肢无负荷大鼠比目鱼肌mTORC1信号传导、MPS和MPB的影响。Sprague-Dawley大鼠(n=12只雄性)随机分为对照组(CON)和后肢无负荷组(HU)。2周后,经皮刺激右比目鱼肌并进行轴上等长收缩。左比目鱼肌起内部控制作用。ES后6小时,我们采集比目鱼肌样本。两周的HU降低了p70S6K和S6rp的激活,这是mTORC1信号传导的下游因素,SUnSET方法评估了MPS,但增加了LC3-II/I比率,这是自噬的指标。废用肌肉上的ES成功激活mTORC1信号,但不影响MPS。相反,ES降低了泛素化蛋白的表达和LC3B-II/I比率。HU可能对急性ES的mTORC1激活和MPS产生不同的影响,这可能是由于ES引起的过量ROS产生。我们的研究结果表明,应用于废弃骨骼肌的ES可能会抑制MPB,但其对MPS的影响似乎减弱了。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Acute effect of electrical stimulation on muscle protein synthesis and break-down in the soleus muscle of hindlimb unloaded rats.

Electrical stimulation (ES) is effective for disuse-induced muscle atrophy. However, the acute effect of ES on muscle protein synthesis (MPS) and muscle protein breakdown (MPB) remains unclear. We investigated the effect of a single-session ES treatment on mTORC1 signaling, MPS, and MPB in the soleus muscle of 2-week hindlimb unloaded rats. Sprague Dawley rats (n = 12 male) were randomly divided into control (CON) and hindlimb unloaded (HU) groups. After 2 weeks, the right soleus muscle was percutaneously stimulated and underwent supramaximal isometric contractions. The left soleus muscle served as an internal control. We collected soleus muscle samples 6 h after ES. Two weeks of HU decreased p70S6K and S6rp activation, downstream factors for mTORC1 signaling, and SUnSET method-assessed MPS, but increased the LC3-II/I ratio, an indicator of autophagy. ES on disused muscle successfully activated mTORC1 signaling but did not affect MPS. Contrary, ES decreased ubiquitinated proteins expression and LC3B-II/I ratio. HU might affect mTORC1 activation and MPS differently in response to acute ES possibly due to excessive ROS production caused by ES. Our findings suggest that ES applied to disused skeletal muscles may suppress MPB, but its effect on MPS appears to be attenuated.

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来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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