胰岛素抵抗对骨骼肌胰岛素抵抗肌管模型中细胞外BCAA积累和SLC25A44表达的影响。

IF 3 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Amino Acids Pub Date : 2023-09-23 DOI:10.1007/s00726-023-03336-8

Caroline N. Rivera, Rachel M. Watne, Andrew J. Wommack, Roger A. Vaughan

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引用次数: 0

摘要

胰岛素抵抗通常伴有循环支链氨基酸（BCAA）升高。我们使用胰岛素抵抗的肌管模型研究了胰岛素抵抗对线粒体BCAA转运蛋白SLC25A44的影响。通过蛋白质印迹评估胰岛素敏感性和SLC25A44表达。液相色谱-质谱法用于评价细胞外BCAA培养基的含量。胰岛素抵抗降低了胰岛素刺激后pAkt的激活，但没有改变SLC25A44的表达。在选定的条件下，胰岛素抵抗导致细胞外BCAA的积累。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The effect of insulin resistance on extracellular BCAA accumulation and SLC25A44 expression in a myotube model of skeletal muscle insulin resistance

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The effect of insulin resistance on extracellular BCAA accumulation and SLC25A44 expression in a myotube model of skeletal muscle insulin resistance

Insulin resistance is often accompanied by elevated circulating branched-chain amino acids (BCAA). We investigated the effects of insulin resistance on the mitochondrial BCAA transporter, SLC25A44, using a myotube model of insulin resistance. Insulin sensitivity and SLC25A44 expression were assessed via Western blot. Liquid chromatography-mass spectrometry was used to evaluate extracellular BCAA media content. Insulin resistance reduced pAkt activation following insulin stimulation but did not alter SLC25A44 expression. Under select conditions, insulin resistance led to the accumulation of extracellular BCAA.

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来源期刊

Amino Acids 生物-生化与分子生物学

CiteScore

6.40

自引率

5.70%

发文量

审稿时长

2.2 months

期刊介绍： Amino Acids publishes contributions from all fields of amino acid and protein research: analysis, separation, synthesis, biosynthesis, cross linking amino acids, racemization/enantiomers, modification of amino acids as phosphorylation, methylation, acetylation, glycosylation and nonenzymatic glycosylation, new roles for amino acids in physiology and pathophysiology, biology, amino acid analogues and derivatives, polyamines, radiated amino acids, peptides, stable isotopes and isotopes of amino acids. Applications in medicine, food chemistry, nutrition, gastroenterology, nephrology, neurochemistry, pharmacology, excitatory amino acids are just some of the topics covered. Fields of interest include: Biochemistry, food chemistry, nutrition, neurology, psychiatry, pharmacology, nephrology, gastroenterology, microbiology