厚高尔基体星形细胞过程的高压电镜三维形态分析。

Kiyoshi Hama, Tatsuo Arii, Eisaku Katayama, Maryann Marton, Mark H Ellisman
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引用次数: 55

摘要

星形胶质细胞过程的一个特征是呈现其他脑成分的胫层或片状覆盖物的形式。利用倾斜高压电子显微镜图像,利用计算机电子断层扫描和立体摄影测量技术,对大鼠齿状回分子层原生质星形胶质细胞的广泛而精细的分布过程进行了分析。测量了星形细胞过程的表面积和体积,并估计了表面/体积比。神经堆星形细胞突起的表面/体积比为18.9 ~ 33.0 / μ m,平均值为26.2+/-5.0 / μ m。这些值与先前报道的大鼠小脑伯格曼胶质细胞末端过程的微域大致相当,这些微域是通过薄连续切片电镜图像重建估计的。神经堆中星形胶质细胞突的大表面积体积比是由于其夹在其他细胞元件之间的层状性质,可能反映了星形胶质细胞的功能活动。结果表明,电子断层扫描和立体摄影测量对星形细胞过程的三维形态测量分析是有用的,尽管这两种技术都可以进一步改进,以提供这些复杂过程的更精确的测量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tri-dimensional morphometric analysis of astrocytic processes with high voltage electron microscopy of thick Golgi preparations.

A characteristic feature of the astrocytic processes is to assume the form of shin sheets or lamellate coverings of other brain constituents. We analyzed the extensive and finely divided processes of the protoplasmic astrocyte in the molecular layer of the rat dentate gyrus by means of computer electron tomography and stereo-photogrammetry using tilted high voltage electron microscope images of thick Golgi preparations. The surface area and volume of the astrocytic processes were measured and the surface/volume ratios were estimated. The surface/volume ratios of astrocytic processes in the neuropile ranged from 18.9 to 33.0 per microm, and the mean value was 26.2+/-5.0 per microm. The values were roughly comparable to those previously reported for the microdomain of Bergmann glia cell terminal processes in the rat cerebellum, which were estimated from reconstructions using thin serial section electron microscope images. The large surface to volume ratio of the astrocytic processes in the neuropile resulted from the lamellar nature of the processes interposed between other cellular elements, and may reflect the functional activities of the astrocyte. The results suggest the usefulness of the electron tomography and stereo-photogrammetry for three-dimensional morphometrical analysis of the astrocytic processes, although both techniques can be expected to be refined further in order to provide more precise measurements of these complicated processes.

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