利用新型荧光核苷的荧光变化清晰区分互补链上的嘧啶碱基。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI:10.1093/nass/3.1.171

Kazuo Tanaka, Akimitsu Okamoto, Isao Saito

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引用次数: 1

摘要

新的荧光核苷，甲氧基苯并二氮杂腺嘌呤((MD)A)和甲氧基苯并二氮杂苷((MD)I)，分别能明显区分C和T碱基。含有(MD)A和(MD)I的ODN探针与靶DNA杂交，便于利用荧光光谱判断靶DNA上特定位点的嘧啶碱基类型。含有ODN的(MD)A-和(MD)I是非常有效的嘧啶SNP分型探针。

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Clear distinction of pyrimidine bases on the complementary strand by fluorescence change of novel fluorescent nucleosides.

New fluorescent nucleosides, methoxybenzodeazaadenine ((MD)A) and methoxybenzodeazainosine ((MD)I) which can sharply distinguish between C and T bases, respectably. The hybridization of an ODN probe containing (MD)A and (MD)I with a target DNA facilitates the judgment with the fluorescence spectra of the type of pyrimidine bases located at a specific site on the target DNA. The (MD)A- and (MD)I containing ODN are very effective probes for pyrimidine SNP typing.

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Nucleic acids research. Supplement (2001)

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