{"title":"荧光显微镜观察组蛋白对玻璃上拉伸DNA的切割作用。","authors":"Yoshihiro Takeda, Fumitaka Mafuné, Tamotsu Kondow","doi":"10.1093/nass/3.1.187","DOIUrl":null,"url":null,"abstract":"<p><p>A stretched single lambda DNA molecule labeled with TOTO-1 dye molecules was immobilized on a cover slip by casting a solution of DNA-lipid polyion complex containing TOTO-1 dye, and were loaded with an aqueous solution of histone. Fluorescence microscopy applied to the single DNA showed that (1) the TOTO-1 dye molecules intercalated in the stretched DNA molecule were vertically aligned almost completely with respect to the DNA axis, and (2) the stretched DNA was cleaved by interaction with histone molecules.</p>","PeriodicalId":86149,"journal":{"name":"Nucleic acids research. Supplement (2001)","volume":" 3","pages":"187-8"},"PeriodicalIF":0.0000,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/3.1.187","citationCount":"0","resultStr":"{\"title\":\"Cleavage of stretched DNA on glass by histones observed with fluorescence microscopy.\",\"authors\":\"Yoshihiro Takeda, Fumitaka Mafuné, Tamotsu Kondow\",\"doi\":\"10.1093/nass/3.1.187\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A stretched single lambda DNA molecule labeled with TOTO-1 dye molecules was immobilized on a cover slip by casting a solution of DNA-lipid polyion complex containing TOTO-1 dye, and were loaded with an aqueous solution of histone. Fluorescence microscopy applied to the single DNA showed that (1) the TOTO-1 dye molecules intercalated in the stretched DNA molecule were vertically aligned almost completely with respect to the DNA axis, and (2) the stretched DNA was cleaved by interaction with histone molecules.</p>\",\"PeriodicalId\":86149,\"journal\":{\"name\":\"Nucleic acids research. Supplement (2001)\",\"volume\":\" 3\",\"pages\":\"187-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1093/nass/3.1.187\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nucleic acids research. Supplement (2001)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1093/nass/3.1.187\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nucleic acids research. Supplement (2001)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/nass/3.1.187","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cleavage of stretched DNA on glass by histones observed with fluorescence microscopy.
A stretched single lambda DNA molecule labeled with TOTO-1 dye molecules was immobilized on a cover slip by casting a solution of DNA-lipid polyion complex containing TOTO-1 dye, and were loaded with an aqueous solution of histone. Fluorescence microscopy applied to the single DNA showed that (1) the TOTO-1 dye molecules intercalated in the stretched DNA molecule were vertically aligned almost completely with respect to the DNA axis, and (2) the stretched DNA was cleaved by interaction with histone molecules.
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