辣椒(Capsicum annuum L.) 12-氧植物烯酸还原酶(OPR)基因家族的鉴定及CaOPR6在辣椒果实发育和胁迫响应中的功能表征

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2022-11-01 Epub Date: 2022-08-09 DOI:10.1139/gen-2022-0037
Wen-Feng Nie, Yue Chen, Junjie Tao, Yu Li, Jianping Liu, Yong Zhou, Youxin Yang
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引用次数: 0

摘要

12-氧植物烯酸还原酶(OPR)是类十八烷生物合成途径中决定茉莉酸生物合成的一种酶。虽然opr在几种作物中的作用已被广泛研究,但对opr编码基因在辣椒植物中的生物学功能知之甚少。本研究从玉米基因组中鉴定出7个OPR家族基因(CaOPR1-7)。进一步分析CaOPR1-7的理化性质,包括基因表达模式、启动子元件和染色体位置。结果表明,7个CaOPR同源物可分为两个亚群,CaOPR6在拟南芥中与AtOPR3高度相似。CaOPR6的表达在低温、盐胁迫、病原菌感染等多种胁迫下均受到显著诱导,表明CaOPR6在应对非生物胁迫和生物胁迫中发挥重要作用。总的来说,这些发现提高了对CaOPR6在辣椒果实发育和辣椒植物胁迫反应中的生物学功能的认识,并为进一步研究茄科蔬菜中OPR蛋白的分子生物学研究提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of the 12-oxophytoeienoic acid reductase (OPR) gene family in pepper (Capsicum annuum L.) and functional characterization of CaOPR6 in pepper fruit development and stress response.

The 12-oxophytoeienoic acid reductase (OPR) is a kind of enzyme in the octadecanoid biosynthesis pathway that determines the biosynthesis of jasmonic acid. Although the roles of OPRs have been extensively studied in several crop plants, little is known about the biological functions of OPR-encoding genes in Capsicum annuum plants. In this study, seven OPR family genes (CaOPR1-7) were identified from the C. annuum genome. The physical and chemical properties of CaOPR1-7 were further analyzed, including gene expression patterns, promoter elements, and chromosomal locations. The results showed that the seven CaOPR homologues could be divided into two subgroups, and CaOPR6 was highly similar to AtOPR3 in Arabidopsis. The expression of CaOPR6 was significantly induced by various stresses such as cold, salt, and pathogen infection, indicating that CaOPR6 plays important roles in response to abiotic and biotic stresses. Overall, these findings improve the understanding of the biological functions of CaOPR6 in the development of pepper fruit and stress response of pepper plants, and facilitate further studies on the molecular biology of OPR proteins in Solanaceae vegetables.

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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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