分子梳理及其在临床中的应用。

IF 1.3 4区生物学 Q4 GENETICS & HEREDITY

Molecular Cytogenetics Pub Date : 2022-11-16 DOI:10.1186/s13039-022-00628-8

Yiping Wang, Kishore Ramesh Kumar, Thomas Liehr

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引用次数: 0

摘要

分子梳理技术(MCT)是拉伸DNA分子并使其易于原位研究的有效手段。MCT利用液体流动过程中施加的力，通过表面张力拉伸DNA分子，并将其扩散到固体表面，即玻璃盖卡瓦。许多DNA分子可以同时平行拉伸，并排整齐排列，使该方法便于统计分析。因此，DNA复制和转录可以在单分子水平上进行研究。本文介绍和讨论了MCT的原理、实验方法、重要应用、在医学领域的优势和捷径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Molecular combing and its application in clinical settings.

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Molecular combing and its application in clinical settings.

Molecular combing technology (MCT) is an effective means for stretching DNA molecules and making them thus accessible for in situ studies. MCT uses the force exerted in the process of liquid flow via surface tension to stretch DNA molecules and spread them on solid surfaces, i.e. glass cover slips. Many DNA molecules can be stretched at the same time in parallel and neatly arranged side-by-side, making the approach convenient for statistical analysis. Accordingly, DNA replication and transcription can be studied at the single molecule level. In this paper, the principle, experimental methods, important applications, advantages and shortcuts of MCT in medical field are presented and discussed.

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来源期刊

Molecular Cytogenetics GENETICS & HEREDITY-

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Molecular Cytogenetics encompasses all aspects of chromosome biology and the application of molecular cytogenetic techniques in all areas of biology and medicine, including structural and functional organization of the chromosome and nucleus, genome variation, expression and evolution, chromosome abnormalities and genomic variations in medical genetics and tumor genetics. Molecular Cytogenetics primarily defines a large set of the techniques that operate either with the entire genome or with specific targeted DNA sequences. Topical areas include, but are not limited to: -Structural and functional organization of chromosome and nucleus- Genome variation, expression and evolution- Animal and plant molecular cytogenetics and genomics- Chromosome abnormalities and genomic variations in clinical genetics- Applications in preimplantation, pre- and post-natal diagnosis- Applications in the central nervous system, cancer and haematology research- Previously unreported applications of molecular cytogenetic techniques- Development of new techniques or significant enhancements to established techniques. This journal is a source for numerous scientists all over the world, who wish to improve or introduce molecular cytogenetic techniques into their practice.