Hyungjun Park, Yosuke Narasako, Tomoko Abe, Hisato Kunitake, Tomonari Hirano
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Ar-ion irradiation had an especially high biological effect on shoot regeneration. A total of 335 lines were obtained, consisting of 104 and 231 lines derived from Ar- and C-ion irradiation, respectively. The change in the DNA content of the lines was analyzed by flow cytometry to evaluate the irradiation-induced damage to the DNA. The two lines demonstrated significant differences in the DNA content and changes at the chromosome level. The screening for the morphological mutants was conducted in the field. Some irradiated lines showed inhibited or no tuberous root phenotype as mutant candidates. Additionally, the high-yield mutant candidates were dominated by Ar-ion irradiation. 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引用次数: 0
摘要
甘薯是一种主要的根茎作物,具有营养丰富的块根。块根的发育机制尚未得到充分阐明。需要遗传资源来发展对甘薯的分子认识。为了增加遗传变异,对六倍体甘薯应用了重离子束,之后研究了重离子束辐照的综合效应。用剂量为 1-5 Gy 的氩离子和剂量为 5-20 Gy 的丙离子对'Beniharuka'腋芽进行离体培养,并从每个辐照芽中分离出三个辐照品系。在高剂量离子辐照下,嫩枝再生受到抑制。氩离子辐照对嫩枝再生的生物效应特别高。共获得 335 个品系,其中氩离子和氩离子辐照品系分别为 104 个和 231 个。通过流式细胞仪分析了各品系 DNA 含量的变化,以评估辐照对 DNA 造成的损伤。两个品系的 DNA 含量和染色体水平的变化均有显著差异。形态突变体的筛选在田间进行。一些辐照品系表现出块根表型受抑制或无块根表型,成为候选突变体。此外,高产突变体候选株以氩离子辐照为主。结果表明,重离子束诱变可有效扩大六倍体甘薯块根形成表型的范围。
Comprehensive effects of heavy-ion beam irradiation on sweet potato (Ipomoea batatas [L.] Lam.).
Sweet potato is a major root crop with nutritious tuberous roots. The mechanism of tuberous root development has not yet been adequately elucidated. Genetic resources are required to develop the molecular understanding of sweet potato. Heavy-ion beams were applied to hexaploid sweet potato for an increase in genetic variation, after which the comprehensive effects of heavy-ion beam irradiation were investigated. In vitro cultured shoots with an axillary bud of 'Beniharuka' were irradiated with Ar-ions at a dose of 1-5 Gy and C-ions at a dose of 5-20 Gy, and three irradiated lines were separated from each irradiated shoot. The shoot regeneration was inhibited at high doses of each ion irradiation. Ar-ion irradiation had an especially high biological effect on shoot regeneration. A total of 335 lines were obtained, consisting of 104 and 231 lines derived from Ar- and C-ion irradiation, respectively. The change in the DNA content of the lines was analyzed by flow cytometry to evaluate the irradiation-induced damage to the DNA. The two lines demonstrated significant differences in the DNA content and changes at the chromosome level. The screening for the morphological mutants was conducted in the field. Some irradiated lines showed inhibited or no tuberous root phenotype as mutant candidates. Additionally, the high-yield mutant candidates were dominated by Ar-ion irradiation. It was indicated that heavy-ion beam mutagenesis is effective in broadening the range of the phenotypes corresponding to tuberous root formation in hexaploid sweet potato.
期刊介绍:
Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.