百里醌通过降低内质网应激、炎症和细胞凋亡减轻阿霉素诱导的急性肾损伤。

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnic & Histochemistry Pub Date : 2022-11-01 Epub Date: 2022-08-22 DOI:10.1080/10520295.2022.2111465
Emin Kaymak, Emel Öztürk, Ali Tuğrul Akİn, Derya Karabulut, Birkan Yakan
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引用次数: 5

摘要

阿霉素(DOX)被用作抗癌药物,尽管它有许多副作用。百里醌(THQ)是一种植物源性物质,具有抗氧化和抗炎特性。我们研究了THQ对DOX所致大鼠肾毒性的保护作用。将大鼠分为5组,每组8只:1组为未处理对照组;第2组,橄榄油组,腹腔灌胃橄榄油14 d;第3组,四氢大麻素组给予10 mg/kg四氢大麻素,ig给药14 d;第4组,DOX组在试验第7天给予单剂量DOX,每次15 mg/kg;第5组,DOX + THQ给药10 mg/kg THQ,第14天,第7天给药15 mg/kg DOX。对肾组织进行组织病理学检查。采用Caspase-3、IL-17、GRP78、TNF-α免疫染色法检测各组间这些蛋白的表达水平。采用TUNEL法测定细胞凋亡指数。采用ELISA法检测肾组织总抗氧化状态(TAS)、总氧化状态(TOS)及TNF-α、TGF-β1水平。4组组织病理损伤、caspase-3、IL-17、GRP78、TNF-α免疫反应性、TUNEL阳性细胞、TOS、TNF-α、TGF-β1水平均较1组升高。4组TAS较1组降低。我们发现,与4组相比,5组caspase-3、IL-17、GRP78和TNF-α的表达降低,TUNEL阳性细胞增多。在给予DOX的大鼠中,THQ通过抑制内质网应激、炎症和凋亡途径来减轻肾损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Thymoquinone alleviates doxorubicin induced acute kidney injury by decreasing endoplasmic reticulum stress, inflammation and apoptosis.

Doxorubicin (DOX) is used as an anticancer drug despite its many side effects. Thymoquinone (THQ) is a plant-derived substance that exhibits antioxidant and anti-inflammatory properties. We investigated the protective effects of THQ on DOX induced nephrotoxicity in rats. Rats were divided into five groups of eight: group 1, untreated control; group 2, olive oil group given olive oil intraperitoneally (i.p.) for 14 days; group 3, THQ group given 10 mg/kg THQ i.p. for 14 days; group 4, DOX group given a single dose of 15 mg/kg DOX i.p. on day 7 of experiment; group 5, DOX + THQ given 10 mg/kg THQ i.p. for 14 days and 15 mg/kg DOX i.p. on day 7. Kidney tissues were evaluated for histopathology. Caspase-3, IL-17, GRP78 and TNF-α immunostaining was used to determine the expression levels of these proteins among the groups. The TUNEL method was used to determine the apoptotic index. Total antioxidant status (TAS), total oxidant status (TOS), and TNF-α and TGF-β1 levels in kidney tissue were measured using ELISA assay. Histopathologic damage, caspase-3, IL-17, GRP78 and TNF-α immunoreactivity, TUNEL positive cells, TOS, TNF-α and TGF-β1 levels were increased in group 4 compared to group 1. The TAS of group 4 decreased compared to group 1. We found decreased caspase-3, IL-17, GRP78 and TNF-α expressions and TUNEL positive cells in group 5 compared to group 4. In rats given DOX, THQ reduced kidney damage by suppressing endoplasmic reticulum stress, inflammation and apoptosis pathways.

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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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