{"title":"基质辅助激光解吸/电离飞行时间质谱(MALDI TOF MS)和VITEK 2在常规微生物鉴定中的评价。","authors":"Anitha Madhavan, Arun Sachu, Nandini Sethuraman, Anil Kumar, Jayalakshmi Vasudevapanicker","doi":"10.4314/gmj.v55i4.12","DOIUrl":null,"url":null,"abstract":"<p><strong>Bacground: </strong>Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories.</p><p><strong>Objectives: </strong>To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2 in routine microbial identification.</p><p><strong>Methods: </strong>The performances of MALDI-TOF MS and VITEK 2 were compared for identifying microorganisms.</p><p><strong>Results: </strong>MALDI- TOF MS and VITEK-2 correctly identified 96 % (96/100) and 97% (97/100) of the isolates upto the genus level.</p><p><strong>Conclusion: </strong>MALDI TOF MS and VITEK -2 gave comparable identification and error rates. The rapid reduction in turnaround time with MALDI TOF is a significant game-changer in the field of clinical microbiology.</p><p><strong>Funding: </strong>State Board of Medical Research (SBMR).</p>","PeriodicalId":35509,"journal":{"name":"Ghana Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334958/pdf/","citationCount":"0","resultStr":"{\"title\":\"Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification.\",\"authors\":\"Anitha Madhavan, Arun Sachu, Nandini Sethuraman, Anil Kumar, Jayalakshmi Vasudevapanicker\",\"doi\":\"10.4314/gmj.v55i4.12\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Bacground: </strong>Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories.</p><p><strong>Objectives: </strong>To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2 in routine microbial identification.</p><p><strong>Methods: </strong>The performances of MALDI-TOF MS and VITEK 2 were compared for identifying microorganisms.</p><p><strong>Results: </strong>MALDI- TOF MS and VITEK-2 correctly identified 96 % (96/100) and 97% (97/100) of the isolates upto the genus level.</p><p><strong>Conclusion: </strong>MALDI TOF MS and VITEK -2 gave comparable identification and error rates. The rapid reduction in turnaround time with MALDI TOF is a significant game-changer in the field of clinical microbiology.</p><p><strong>Funding: </strong>State Board of Medical Research (SBMR).</p>\",\"PeriodicalId\":35509,\"journal\":{\"name\":\"Ghana Medical Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334958/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ghana Medical Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4314/gmj.v55i4.12\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ghana Medical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/gmj.v55i4.12","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification.
Bacground: Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories.
Objectives: To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2 in routine microbial identification.
Methods: The performances of MALDI-TOF MS and VITEK 2 were compared for identifying microorganisms.
Results: MALDI- TOF MS and VITEK-2 correctly identified 96 % (96/100) and 97% (97/100) of the isolates upto the genus level.
Conclusion: MALDI TOF MS and VITEK -2 gave comparable identification and error rates. The rapid reduction in turnaround time with MALDI TOF is a significant game-changer in the field of clinical microbiology.
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