7-氨基-4-甲基香豆素作为希夫试剂的荧光替代品:一种可以在同一组织切片上与血凝和伊红染色相结合的新方法。

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnic & Histochemistry Pub Date : 2023-01-01 Epub Date: 2022-07-29 DOI:10.1080/10520295.2022.2101144
Hiroshi Takase, Takayuki Murase, Daisuke Hachisuka, Yuma Sakamoto, Mariko Sugiura, Satsuki Nakano, Keiichiro Fujii, Ayako Masaki, Hiroshi Inagaki
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引用次数: 2

摘要

7-氨基-4-甲基香豆素(AMC)水溶液在紫外(UV)光下表现出强荧光,可作为希夫试剂可视化醛类。采用血红、伊红(h&e)和AMC染色进行组织病理分析。采用h&e /AMC联合染色法对正常和病变人体组织切片进行染色。h&e /AMC染色后,明场显微镜下保存h&e形态。紫外光下观察到AMC荧光信号强烈,染色模式与PAS (periodic acid-Schiff)染色相同。存档的h&e切片的AMC染色也很成功。淀粉酶消化将糖原与其他AMC阳性元素区分开来。利用H & E/AMC染色,在紫外激发下清晰可见富黏液的腺癌细胞、阿米巴滋养体和真菌菌丝。采用h&e /AMC染色,单张组织切片即可获得h&e和pas样组织学成像。h&e /AMC对于病理诊断是有用的,特别是当PAS染色的信息是关键的,组织切片的数量有限和/或所讨论的病变很小。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
7-Amino-4-methylcoumarin as a fluorescent substitute for Schiff's reagent: a new method that can be combined with hemalum and eosin staining on the same tissue section.

An aqueous 7-amino-4-methylcoumarin (AMC) solution exhibits strong fluorescence under ultraviolet (UV) light and can be used as a Schiff reagent to visualize aldehydes. We investigated hemalum and eosin (H & E) and AMC staining for histological and pathological analysis. Sections of normal and lesioned human tissues were stained with combined H & E/AMC staining. After H & E/AMC staining, the H & E morphology was preserved under bright field microscopy. The AMC fluorescent signals observed under UV light were intense and the staining pattern was identical to that obtained by periodic acid-Schiff (PAS) staining. AMC staining of archived H & E sections also was successful. Diastase digestion differentiated glycogen from other AMC positive elements. Using H & E/AMC staining, mucus-rich adenocarcinoma cells, amebic trophozoites and fungal hyphae were visualized clearly under UV excitation. Using H & E/AMC staining, H & E and PAS-like histological imaging can be obtained using a single tissue section. H & E/AMC is useful for pathologic diagnosis especially when information from PAS staining is critical, the number of tissue sections is limited and/or the lesion in question is small.

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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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