Fan Li, Yun Wang, Hui Yang, Yingying Xu, Xiaoyan Zhou, Xiao Zhang, Zhaohong Xie, Jianzhong Bi
{"title":"BACE1- as通过调节BACE1 mRNA表达对β-淀粉样蛋白生成的影响","authors":"Fan Li, Yun Wang, Hui Yang, Yingying Xu, Xiaoyan Zhou, Xiao Zhang, Zhaohong Xie, Jianzhong Bi","doi":"10.1186/s12867-019-0140-0","DOIUrl":null,"url":null,"abstract":"<p>The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1 mRNA and promotes the formation of Aβ. After the administration of Aβ<sub>1-42</sub> to SH-SY5Y cells and C57/BL6J mice, we detected the expression of BACE1-AS, BACE1 mRNA, and BACE1 protein, as well as the concentration of Aβ<sub>1-40</sub>. Then, we silenced the expression of BACE1-AS in SH-SY5Y and 20E2 cells using siRNAs targeting BACE1-AS and detected its effects on the levels of the BACE1 mRNA and BACE1 protein and Aβ<sub>1-40</sub> generation.</p><p>The administration of Aβ<sub>1-42</sub> increased the expression of BACE1-AS, BACE1 mRNA and protein, as well as the concentration of Aβ<sub>1-40</sub> in SH-SY5Y cells and the brains of C57BL/6J mice. Pretreatment with the BACE1-AS siRNA inhibited the effect of Aβ<sub>1-42</sub> on increasing the expression of BACE1-AS and BACE1, as well as the generation of Aβ.</p><p>The mechanism by which exogenous Aβ<sub>1-42</sub> induces BACE1 expression and Aβ generation is mediated by BACE1-AS. BACE1-AS is involved in the mechanism regulating BACE1 expression and Aβ generation in APPsw transgenic cells.</p>","PeriodicalId":497,"journal":{"name":"BMC Molecular Biology","volume":"20 1","pages":""},"PeriodicalIF":2.9460,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12867-019-0140-0","citationCount":"24","resultStr":"{\"title\":\"The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression\",\"authors\":\"Fan Li, Yun Wang, Hui Yang, Yingying Xu, Xiaoyan Zhou, Xiao Zhang, Zhaohong Xie, Jianzhong Bi\",\"doi\":\"10.1186/s12867-019-0140-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1 mRNA and promotes the formation of Aβ. After the administration of Aβ<sub>1-42</sub> to SH-SY5Y cells and C57/BL6J mice, we detected the expression of BACE1-AS, BACE1 mRNA, and BACE1 protein, as well as the concentration of Aβ<sub>1-40</sub>. Then, we silenced the expression of BACE1-AS in SH-SY5Y and 20E2 cells using siRNAs targeting BACE1-AS and detected its effects on the levels of the BACE1 mRNA and BACE1 protein and Aβ<sub>1-40</sub> generation.</p><p>The administration of Aβ<sub>1-42</sub> increased the expression of BACE1-AS, BACE1 mRNA and protein, as well as the concentration of Aβ<sub>1-40</sub> in SH-SY5Y cells and the brains of C57BL/6J mice. Pretreatment with the BACE1-AS siRNA inhibited the effect of Aβ<sub>1-42</sub> on increasing the expression of BACE1-AS and BACE1, as well as the generation of Aβ.</p><p>The mechanism by which exogenous Aβ<sub>1-42</sub> induces BACE1 expression and Aβ generation is mediated by BACE1-AS. BACE1-AS is involved in the mechanism regulating BACE1 expression and Aβ generation in APPsw transgenic cells.</p>\",\"PeriodicalId\":497,\"journal\":{\"name\":\"BMC Molecular Biology\",\"volume\":\"20 1\",\"pages\":\"\"},\"PeriodicalIF\":2.9460,\"publicationDate\":\"2019-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1186/s12867-019-0140-0\",\"citationCount\":\"24\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Molecular Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s12867-019-0140-0\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1186/s12867-019-0140-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1 mRNA and promotes the formation of Aβ. After the administration of Aβ1-42 to SH-SY5Y cells and C57/BL6J mice, we detected the expression of BACE1-AS, BACE1 mRNA, and BACE1 protein, as well as the concentration of Aβ1-40. Then, we silenced the expression of BACE1-AS in SH-SY5Y and 20E2 cells using siRNAs targeting BACE1-AS and detected its effects on the levels of the BACE1 mRNA and BACE1 protein and Aβ1-40 generation.
The administration of Aβ1-42 increased the expression of BACE1-AS, BACE1 mRNA and protein, as well as the concentration of Aβ1-40 in SH-SY5Y cells and the brains of C57BL/6J mice. Pretreatment with the BACE1-AS siRNA inhibited the effect of Aβ1-42 on increasing the expression of BACE1-AS and BACE1, as well as the generation of Aβ.
The mechanism by which exogenous Aβ1-42 induces BACE1 expression and Aβ generation is mediated by BACE1-AS. BACE1-AS is involved in the mechanism regulating BACE1 expression and Aβ generation in APPsw transgenic cells.
期刊介绍：
BMC Molecular Biology is an open access journal publishing original peer-reviewed research articles in all aspects of DNA and RNA in a cellular context, encompassing investigations of chromatin, replication, recombination, mutation, repair, transcription, translation and RNA processing and function.
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