{"title":"等位基因特异性聚合酶链反应(AS-PCR)检测超照按蚊乙酰胆碱酯酶敲低和突变","authors":"Sare İlknur Yavaşoğlu, Fatih Mehmet Şimşek","doi":"10.4274/tpd.galenos.2022.20592","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The study aims to determine the presence of L1014F, L1014S, L1014C alleles, which are responsible for knockdown resistance and <i>Ace-1</i> G119S alleles, which are responsible for acetylcholinesterase insensitivity in <i>Anopheles superpictus</i>, the secondary vector of malaria in Turkey.</p><p><strong>Methods: </strong>In this study, 60 <i>Anopheles superpictus</i> adult females were collected from Aydın, Denizli, and Muğla provinces. Then, allele-specific primers for <i>kdr</i> L1014F, L1014S, and L1014C alleles, and the <i>Ace-1</i> G119S allele were designed. The presence of these alleles was screened in three <i>Anopheles superpictus</i> populations by allele-specific polymerase chain reaction.</p><p><strong>Results: </strong>Although L1014S allele frequency was too low in Aydın, Muğla, and Denizli populations, neither <i>kdr</i> L1014F and L1014C nor <i>Ace-1</i> G119S mutations were found in any population.</p><p><strong>Conclusion: </strong>In this study, <i>kdr</i> L1014S mutation was detected for the first time in the Aegean <i>Anopheles superpictus</i> populations.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":" ","pages":"307-311"},"PeriodicalIF":0.0000,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Allele Specific Polymerase Chain Reaction (AS-PCR) Assay to Detect Knockdown and Acetylcholinesterase Mutations in <i>Anopheles superpictus</i>\",\"authors\":\"Sare İlknur Yavaşoğlu, Fatih Mehmet Şimşek\",\"doi\":\"10.4274/tpd.galenos.2022.20592\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The study aims to determine the presence of L1014F, L1014S, L1014C alleles, which are responsible for knockdown resistance and <i>Ace-1</i> G119S alleles, which are responsible for acetylcholinesterase insensitivity in <i>Anopheles superpictus</i>, the secondary vector of malaria in Turkey.</p><p><strong>Methods: </strong>In this study, 60 <i>Anopheles superpictus</i> adult females were collected from Aydın, Denizli, and Muğla provinces. Then, allele-specific primers for <i>kdr</i> L1014F, L1014S, and L1014C alleles, and the <i>Ace-1</i> G119S allele were designed. The presence of these alleles was screened in three <i>Anopheles superpictus</i> populations by allele-specific polymerase chain reaction.</p><p><strong>Results: </strong>Although L1014S allele frequency was too low in Aydın, Muğla, and Denizli populations, neither <i>kdr</i> L1014F and L1014C nor <i>Ace-1</i> G119S mutations were found in any population.</p><p><strong>Conclusion: </strong>In this study, <i>kdr</i> L1014S mutation was detected for the first time in the Aegean <i>Anopheles superpictus</i> populations.</p>\",\"PeriodicalId\":34974,\"journal\":{\"name\":\"Turkiye parazitolojii dergisi\",\"volume\":\" \",\"pages\":\"307-311\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Turkiye parazitolojii dergisi\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4274/tpd.galenos.2022.20592\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkiye parazitolojii dergisi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4274/tpd.galenos.2022.20592","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Allele Specific Polymerase Chain Reaction (AS-PCR) Assay to Detect Knockdown and Acetylcholinesterase Mutations in Anopheles superpictus
Objective: The study aims to determine the presence of L1014F, L1014S, L1014C alleles, which are responsible for knockdown resistance and Ace-1 G119S alleles, which are responsible for acetylcholinesterase insensitivity in Anopheles superpictus, the secondary vector of malaria in Turkey.
Methods: In this study, 60 Anopheles superpictus adult females were collected from Aydın, Denizli, and Muğla provinces. Then, allele-specific primers for kdr L1014F, L1014S, and L1014C alleles, and the Ace-1 G119S allele were designed. The presence of these alleles was screened in three Anopheles superpictus populations by allele-specific polymerase chain reaction.
Results: Although L1014S allele frequency was too low in Aydın, Muğla, and Denizli populations, neither kdr L1014F and L1014C nor Ace-1 G119S mutations were found in any population.
Conclusion: In this study, kdr L1014S mutation was detected for the first time in the Aegean Anopheles superpictus populations.
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