类器官成像:观察发育和功能。

IF 11.4 1区生物学 Q1 CELL BIOLOGY

Annual review of cell and developmental biology Pub Date : 2022-10-06 Epub Date: 2022-06-29 DOI:10.1146/annurev-cellbio-120320-035146

Rashmiparvathi Keshara, Yung Hae Kim, Anne Grapin-Botton

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引用次数: 6

摘要

类器官是在体外从干细胞或祖细胞中产生的器官的小型化和简化版本。它们被用作由多种细胞类型组成的模型系统，形成与器官相关的结构并执行器官的功能。它们是研究发育、体内平衡、再生和疾病的有用工具。类器官成像已成为可视化和理解其自组织、对称破缺、生长、分化和功能的关键方法。在这篇综述中，我们讨论了成像方法，如何分析这些图像，以及类器官研究面临的挑战。

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Organoid Imaging: Seeing Development and Function.

Organoids are miniaturized and simplified versions of an organ produced in vitro from stem or progenitor cells. They are used as a model system consisting of multiple cell types forming an architecture relevant to the organ and carrying out the function of the organ. They are a useful tool to study development, homeostasis, regeneration, and disease. The imaging of organoids has become a pivotal method to visualize and understand their self-organization, symmetry breaking, growth, differentiation, and function. In this review, we discuss imaging methods, how to analyze these images, and challenges in organoid research.

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来源期刊

Annual review of cell and developmental biology 生物-发育生物学

CiteScore

19.50

自引率

0.00%

发文量

期刊介绍： The Annual Review of Cell and Developmental Biology, established in 1985, comprehensively addresses major advancements in cell and developmental biology. Encompassing the structure, function, and organization of cells, as well as the development and evolution of cells in relation to both single and multicellular organisms, the journal explores models and tools of molecular biology. As of the current volume, the journal has transitioned from gated to open access through Annual Reviews' Subscribe to Open program, making all articles published under a CC BY license.