Mahdieh Raeeszadeh, Seed Mohammad Saleh Hosseini, Ali Akbar Amiri
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The main measurements included total antioxidant capacity (TAC), glutathione peroxidase (GPx), malondialdehyde (MDA), interleukin 8 (IL-8), tumor necrosis factor-<i>α</i> (TNF<i>α</i>), follicle stimulating hormone (FSH), luteinizing hormone (LH), and estrogen (ES). Except for the C and T3 groups, the other groups lost weight. A significantly lower concentration of MDA was observed in the T3 group. However, TAC was substantially increased compared to the other groups. The level of GPx in the S group was significantly reduced compared to all groups. Proinflammatory markers (IL-8 and TNF<i>α</i>) reached their lowest serum level in the T3 group, with a statistically significant difference compared to that of the S group. In addition, there were no significant differences in the means of primary, secondary, and graph and atretic follicles between the T3 and C group. On the other hand, a decrease in FSH and LH was observed while an increase in ES was seen in the T3 group compared to the S group. This study revealed that N-acetylcysteine and vitamin E coadministration could significantly decrease the side effects of cyclophosphamide, especially in ovarian tissue.</p>","PeriodicalId":17421,"journal":{"name":"Journal of Toxicology","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2022-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427260/pdf/","citationCount":"9","resultStr":"{\"title\":\"Impact of Co-Administration of N-Acetylcysteine and Vitamin E on Cyclophosphamide-Induced Ovarian Toxicity in Female Rats.\",\"authors\":\"Mahdieh Raeeszadeh, Seed Mohammad Saleh Hosseini, Ali Akbar Amiri\",\"doi\":\"10.1155/2022/9073405\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Cyclophosphamide is used to treat various types of cancer. 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引用次数: 9
摘要
环磷酰胺用于治疗各种类型的癌症。然而,它会降低卵巢功能和生育率。本研究比较了n -乙酰半胱氨酸和维生素E对环磷酰胺引起的卵巢损伤的影响。将35只大鼠随机分为5组:对照组(C)、环磷酰胺(CP, 200 mg/kg单次腹腔注射)、T1组(环磷酰胺+维生素E 200 mg/kg)、T2组(环磷酰胺+ n -乙酰半胱氨酸200 mg/kg)、T3组(环磷酰胺+ n -乙酰半胱氨酸和维生素E 200 mg/kg)。主要测定指标为总抗氧化能力(TAC)、谷胱甘肽过氧化物酶(GPx)、丙二醛(MDA)、白细胞介素8 (IL-8)、肿瘤坏死因子-α (tnf -α)、促卵泡激素(FSH)、促黄体生成素(LH)、雌激素(ES)。除C组和T3组外,其他组体重均有所下降。T3组MDA浓度明显降低。然而,与其他组相比,TAC显著增加。与其他各组相比,S组GPx水平明显降低。促炎标志物IL-8、TNFα在T3组达到最低水平,与S组比较差异有统计学意义。此外,T3组与C组在原发性、继发性、曲性和闭锁卵泡的均值上无显著差异。另一方面,与S组相比,T3组FSH和LH降低,而ES升高。本研究表明,n -乙酰半胱氨酸和维生素E共给药可显著降低环磷酰胺的副作用,特别是对卵巢组织的副作用。
Impact of Co-Administration of N-Acetylcysteine and Vitamin E on Cyclophosphamide-Induced Ovarian Toxicity in Female Rats.
Cyclophosphamide is used to treat various types of cancer. However, it can reduce ovarian function and fertility rate. The current study was done to compare the effects of N-acetylcysteine and vitamin E on cyclophosphamide-induced ovarian damage. Thirty-five rats were randomly divided into 5 groups: control (C), cyclophosphamide (CP, 200 mg/kg single dose intraperitoneally), T1 (cyclophosphamide + vitamin E at 200 mg/kg), T2 (cyclophosphamide + 200 mg/kg N-acetylcysteine), and T3 (cyclophosphamide + N-acetylcysteine and vitamin E at 200 mg/kg). The main measurements included total antioxidant capacity (TAC), glutathione peroxidase (GPx), malondialdehyde (MDA), interleukin 8 (IL-8), tumor necrosis factor-α (TNFα), follicle stimulating hormone (FSH), luteinizing hormone (LH), and estrogen (ES). Except for the C and T3 groups, the other groups lost weight. A significantly lower concentration of MDA was observed in the T3 group. However, TAC was substantially increased compared to the other groups. The level of GPx in the S group was significantly reduced compared to all groups. Proinflammatory markers (IL-8 and TNFα) reached their lowest serum level in the T3 group, with a statistically significant difference compared to that of the S group. In addition, there were no significant differences in the means of primary, secondary, and graph and atretic follicles between the T3 and C group. On the other hand, a decrease in FSH and LH was observed while an increase in ES was seen in the T3 group compared to the S group. This study revealed that N-acetylcysteine and vitamin E coadministration could significantly decrease the side effects of cyclophosphamide, especially in ovarian tissue.
期刊介绍:
Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.