自动识别系统RAISUS对B群链球菌识别准确性的研究

Atsuko Yamada, Yuka Yamagishi, Kaori Tanaka, Haruki Sawamura, Tomoko Ohno, Wakako Naito, Hiroya Tani, Kunitomo Watanabe, Hiroshige Mikamo
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引用次数: 0

摘要

由于我们曾发生过一起用自动鉴定系统RAISUS对B群链球菌(无乳链球菌)进行误鉴定的案例,我们利用本公司分离的GBS标本,对自动鉴定系统RAISUS系统对GBS的鉴定准确性进行了研究。包括标准菌株ATCC13813在内的31株GBS中,鉴定为GBS的菌株有30株,仅有1株被误鉴定为星座链球菌。鉴定29株真性GBS菌株耗时3小时,而误鉴定为星座s型的菌株耗时6小时。由于我们需要超过4个小时的时间来识别错误的GBS菌株,因此已经认识到识别时间变长的趋势。较长的识别时间可能与错误识别有关。制造商对RAISUS的半乳糖和甘油测试进行了改进,从而更好地识别。对于经RAISUS鉴定为星座葡萄球菌的菌株,当临床有较大需要时,可与其他鉴定试剂盒进行复核。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Investigation on the identification accuracy for group B Streptococcus by automated identification system RAISUS].

Since we had experienced a case of misidentification for group B Streptococcus (GBS) (Streptococcus agalactiae) by automated identification system RAISUS, we have investigated the identification accuracy for GBS by automated identification system RAISUS system using our stock isolated of GBS. Among 31 strains including standard strain ATCC13813 of GBS, 30 strains were identified as GBS, while only 1 strain was misidentified as Streptococcus constellatus. We have needed 3 hours to identify 29 true GBS strains, while it has taken 6 hours for misidentified strain as S. constellatus. Since we have needed more than 4 hours for our misidentified strain of GBS, the tendency for identification time to become long has been recognized. Longer identification time might be associated with the misidentification. The improvement for galactose and glycerol tests of RAISUS by the manufacturer has resulted in better identification. As for the strains to be identified as S. constellatus by RAISUS, we might re-check it with other identification kits when there is higher clinical necessity.

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