核酸杂交分析中的寡核苷酸衍生物。3含双功能非核苷酸插入物的寡核苷酸的合成与性质研究。

Bioorganicheskaia khimiia Pub Date : 2012-11-01
M S Kupriushkin, D V Pyshnyĭ
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引用次数: 0

摘要

合成了具有不同官能团位置支链骨架的非核苷酸磷酰胺。所制得的磷酰胺单体含有插入物部分-6-氯-2-甲氧基吖啶,并含有二甲氧基三甲基或叔丁基二甲基硅基保护的羟基残基,用于合成后修饰。合成的低胸苷酸酯在序列的不同位置含有一个或多个修饰单元。定义了修饰寡核苷酸形成的互补双相物的熔融温度和形成的热力学参数(焓和熵的变化)。插入残基的引入导致DNA双链的显著稳定。结果表明,与DNA杂交后,寡核苷酸偶联物中吖啶残基的荧光效率发生了显著变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Oligonucleotide derivatives in the nucleic acid hybridization analysis. III. Synthesis and investigation of properties of oligonucleotides, bearing bifunctional non-nucleotide insert].

Non-nucleotide phosporamidites were synthetized, having branched backbone with different position of functional groups. Obtained phosphoramidite monomers contain intercalator moiety--6-chloro-2-methoxyacridine, and additional hydroxyl residue protected with dimethoxytrityl group or with tert-butyldimethylsilyl group for post-synthetic modification. Synthesized oligothymidilates contain one or more modified units in different positions of sequence. Melting temperature and thermodynamic parameters of formation of complementary duplexes formed by modified oligonucleotides was defined (change in enthalpy and entropy). The introduction of intercalating residue causes a significant stabilization of DNA duplexes. It is shown that the efficiency of the fluorescence of acridine residue in the oligonucleotide conjugate significantly changes upon hybridization with DNA.

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