抗pml - rar α shRNA使早幼粒细胞白血病细胞对全反式维甲酸敏感。

Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research Pub Date : 2012-01-01 Epub Date: 2012-07-13

Nicholas P Casey, Gregory M Woods

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摘要

PML-RARα融合基因在一系列白血病类型中破坏视黄酸分化信号，促进增殖。我们设计了一个shRNA来靶向融合mRNA，并通过慢病毒转导传递shRNA表达盒。该shRNA的递送显著下调了靶mRNA，在蛋白质水平上也有明显的作用。当与ATRA联合使用时，融合基因的下调强烈抑制了NB4 PML细胞系的增殖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid.

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Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid.

The PML-RARα fusion gene disrupts the retinoic acid differentiation signal in a range of leukaemia types, promoting proliferation. We designed a shRNA to target the fusion mRNA, and the shRNA expression cassette was delivered via lentiviral transduction. Delivery of this shRNA significantly down regulated the target mRNA, with effects also evident at the protein level. When combined with ATRA administration, this down regulation of the fusion gene strongly inhibited proliferation in the NB4 PML cell line.

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Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research

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