急性亚硝酸盐暴露诱导草鱼离体血细胞功能障碍和氧化损伤

IF 1.5 4区 农林科学 Q3 FISHERIES
Ting-ting Zhang, Pin Ma, Xiao-yan Yin, Dong-ye Yang, Da-peng Li, Rong Tang
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引用次数: 3

摘要

为了评估亚硝酸盐对草鱼Ctenopharyngodon idella血细胞氧化损伤的影响,将分离的血细胞暴露于亚硝酸盐(0、1、10或100 mg/L)中长达24小时。测定血红蛋白(Hb)和高铁血红蛋白(MetHb)浓度、活性氧(ROS)和丙二醛(MDA)水平、线粒体膜电位(∆Ψm)和抗氧化酶活性,以评估血液学参数和抗氧化防御机制。结果表明,亚硝酸盐暴露24 h后,Hb浓度随亚硝酸盐浓度的增加而显著降低,而甲基苯丙胺浓度在亚硝酸盐暴露组中显著升高。随着亚硝酸盐暴露浓度和时间的增加,ROS、∆Ψm和MDA水平均有不同程度的升高。总抗氧化能力、过氧化氢酶(CAT)活性、谷胱甘肽过氧化物酶(GPx)活性和谷胱甘肽含量均随暴露时间的延长呈现先升高后降低的趋势。超氧化物歧化酶(SOD)活性在1 mg/L亚硝酸盐暴露组高于对照组,在100 mg/L亚硝酸盐暴露组低于对照组。cat、sod1和gpx的相对信使RNA表达率在1和10 mg/L组显著上调,在100 mg/L组下降。由此可见,亚硝酸盐暴露激活了草鱼血细胞的抗氧化防御机制,较高的亚硝酸盐剂量或较长的亚硝酸盐暴露时间会破坏氧化-抗氧化平衡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Acute Nitrite Exposure Induces Dysfunction and Oxidative Damage in Grass Carp Isolated Hemocytes

To evaluate the effects of nitrite on the oxidative damage of blood cells of Grass Carp Ctenopharyngodon idella, the isolated hemocytes were exposed to nitrite (0, 1, 10, or 100 mg/L) for up to 24 h. Hemoglobin (Hb) and methemoglobin (MetHb) concentrations, reactive oxygen species (ROS) and malondialdehyde (MDA) levels, mitochondrial membrane potential (∆Ψm), and antioxidant enzyme activity were assayed to assess hematological parameters and the antioxidant defense mechanism. Results showed a remarkable decrease in Hb concentration with increasing nitrite concentration after a 24-h exposure, while the MetHb concentration increased significantly in nitrite exposure groups. The levels of ROS, ∆Ψm, and MDA increased to varying degrees with increases in nitrite exposure concentration and time. The total antioxidant capacity, catalase (CAT) activity, glutathione peroxidase (GPx) activity, and glutathione content showed a trend of rising initially and then decreasing with prolonged exposure time. Superoxide dismutase (SOD) activity was higher in the 1-mg/L nitrite exposure group and lower in the 100-mg/L group than in the control. The relative messenger RNA expression ratios of cat, sod1, and gpx were up-regulated significantly in the 1- and 10-mg/L groups and then declined in the 100-mg/L group. Therefore, it can be concluded that nitrite exposure activates the antioxidant defense mechanism of Grass Carp hemocytes and that the balance of oxidant–antioxidant homeostasis will be undermined by higher nitrite doses or longer exposure periods.

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来源期刊
Journal of aquatic animal health
Journal of aquatic animal health 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
25
审稿时长
>24 weeks
期刊介绍: The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
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