开菲尔生物膜的表面动力学、分形特征和微观形貌分析

IF 2.1 3区 工程技术 Q2 ANATOMY & MORPHOLOGY
Ştefan Ţălu, Erveton P. Pinto, Robert S. Matos, Amir Zelati, Shahram Solaymani, Azizollah Shafiekhani, Atefeh Ghaderi
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引用次数: 3

摘要

介绍了利用天然新鲜开菲尔颗粒发酵制备巴西莓提取物的生物膜图像分析研究。研究了原子力显微镜数据,旨在了解巴西莓(Euterpe oleracea Mart.)浓度对表面形貌和纹理复杂性的影响,并用分形维数来评估。结果表明,生物膜中巴西莓浓度的增加对生物膜的表面形态和分形维数都有影响。还观察到,当浓度从40到60 ml变化时,生物膜表面呈现饱和状态。另一方面,据观察,用20毫升巴西莓制成的中间样品似乎是生产可作为皮肤敷料的生物膜的最佳点,因为与机械特性和体外和体内试验有关的其他研究可以证实这种适用性。因此,通过表面统计参数和分形几何的评价来表征开菲尔生物膜的表面形态,可能会为这些膜的适用性提供有希望的结果。我们表征了与açaí提取物相关的开菲尔生物膜三维表面的结构复杂性。使用原子力显微镜在接触模式下对样品进行了三维表面分析。测定了分析样品的立体维数和分形维数。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Surface dynamics, fractal features, and micromorphology analysis of kefir biofilms

Surface dynamics, fractal features, and micromorphology analysis of kefir biofilms

We introduce a study of image analysis of kefir biofilms associated with Acai extract prepared by fermentation of fresh kefir grains natural. Atomic force microscopy data were studied, aiming to understand how the concentration of acai berry (Euterpe oleracea Mart.) influences the surface morphology as well as the texture complexity, evaluated by the fractal dimension. The results showed that the superficial morphology was affected by the increase of Acai concentration in the biofilms, as well as the fractal dimension. It has also been observed that the surface of the biofilm presented saturation when concentration changes from 40 to 60 ml. On the other hand, it was observed that the intermediate sample produced with 20 ml of acai berry seems to be the best point for biofilms production that can serve as a skin dressing since other studies related to mechanical properties and in vitro and in vivo tests can confirm this applicability. Thus, the characterization of the surface morphology of kefir biofilms by the evaluation of surface statistical parameters and fractal geometry may provide promising results regarding the applicability of these films.

Research Highlights

  • We characterized the structural complexity of the 3-D surface of the kefir biofilms associated with açaí extract.
  • The 3-D surface analysis of the samples was performed using an atomic force microscope operating in contact mode.
  • We determined the stereometric and fractal dimension of the analyzed samples.
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来源期刊
Microscopy Research and Technique
Microscopy Research and Technique 医学-解剖学与形态学
CiteScore
5.30
自引率
20.00%
发文量
233
审稿时长
4.7 months
期刊介绍: Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.
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