抗gpvi (HY101)在多色流式细胞术中激活血小板。

IF 2.5 3区医学 Q3 CELL BIOLOGY

Platelets Pub Date : 2022-10-03 Epub Date: 2022-01-17 DOI:10.1080/09537104.2022.2027359

Marie-Astrid van Dievoet, Davide Brusa, Marie Octave, Sandrine Horman, Xavier Stephenne

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引用次数: 1

摘要

血小板跨膜受体GPVI可以在全血多色流式细胞术中与其他血小板膜标志物一起评估。在单管中结合多种抗体的优点是可以区分多个血小板亚群。在这篇简短的通信中，我们描述了抗gpvi克隆HY101遇到的激活问题。流式细胞术显示，加入抗gpvi后，血小板活化，活化标记物CD62P、PAC-1、CD63和CD107a的表达突出显示。血小板聚集研究也证实了这一点。

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Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel.

The platelet transmembrane receptor GPVI can be assessed together with other platelet membrane markers in a whole blood multicolor flow cytometry panel. The advantage of combining multiple antibodies in a single tube is the possibility of distinguishing multiple platelet subgroups. In this short communication, we describe an activation problem encountered with anti-GPVI, clone HY101. Activation of platelets was seen after the addition of anti-GPVI in a flow cytometry panel, highlighted by the expression of the activation markers CD62P, PAC-1, CD63, and CD107a. This was also confirmed by platelet aggregation studies.

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来源期刊

Platelets 医学-细胞生物学

CiteScore

6.70

自引率

3.00%

发文量

审稿时长

1 months

期刊介绍： Platelets is an international, peer-reviewed journal covering all aspects of platelet- and megakaryocyte-related research. Platelets provides the opportunity for contributors and readers across scientific disciplines to engage with new information about blood platelets. The journal’s Methods section aims to improve standardization between laboratories and to help researchers replicate difficult methods. Research areas include: Platelet function Biochemistry Signal transduction Pharmacology and therapeutics Interaction with other cells in the blood vessel wall The contribution of platelets and platelet-derived products to health and disease The journal publishes original articles, fast-track articles, review articles, systematic reviews, methods papers, short communications, case reports, opinion articles, commentaries, gene of the issue, and letters to the editor. Platelets operates a single-blind peer review policy. Authors can choose to publish gold open access in this journal.