肉毒毒素A对扩张大鼠皮肤血流的影响。

IF 2.1 4区 医学 Q2 SURGERY
Journal of Investigative Surgery Pub Date : 2022-05-01 Epub Date: 2022-01-10 DOI:10.1080/08941939.2021.1995539
Hengxin Liu, Zhou Yu, Jiayang Wang, Xi Zhang, Lei Lei, Yu Zhang, Yingjun Su, Xianjie Ma
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引用次数: 4

摘要

背景:供血不足易导致扩张皮瓣远端挤压和坏死。肉毒毒素A (BTX-A)可改善带蒂皮瓣血流灌注,但其对扩张皮肤血供的影响尚不清楚。因此,本研究旨在评价BTX-A对扩张期间和扩张后血流灌注的影响。方法18只sd大鼠随机分为BTX-A组和对照组。立即皮内注射BTX-A或生理盐水到背部标记皮肤。然后在大鼠体内植入扩张器。一周后,开始用生理盐水对膨胀器进行充气,每周进行两次,达到囊内压力8 kPa。每次注射前测量皮肤血流量。扩增4周后采集标本进行组织学染色,测量血管直径和密度;同时将2 cm× 8 cm的随机扩张皮瓣抬高原位缝合。观察血流灌注及皮瓣存活情况。结果与对照组相比,BTX-A组扩张皮肤的血流量更多,血管直径更大,血管密度更高。此外,BTX-A组皮瓣在扩大皮瓣移植后7天内血流灌注良好,皮瓣存活面积比例高。数据分析采用独立t检验。结论术前BTX-A治疗可促进血管生成和血管舒张,扩张期间和扩张后血流灌注升高,获得更大比例的移植扩张皮瓣存活面积。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Botulinum Toxin A on the Blood Flow in Expanded Rat Skin.

Background Poor blood supply can easily lead to expander extrusion and necrosis at the distal expanded flap. Botulinum toxin A (BTX-A) has been previously found to improve pedicled flap blood flow perfusion, but its effects on the blood supply of expanded skin remain unclear. Therefore, this study aimed to evaluate the effects of BTX-A on blood flow perfusion during and after expansion.Methods Eighteen Sprague-Dawley rats were randomly divided into a BTX-A group and a control group. BTX-A or normal saline was injected intradermally into the marked skin on the back immediately. Then expanders were implanted in the rats. One week later, inflation of the expander with normal saline was started and performed twice a week to reach an intracapsular pressure of 8 kPa. The skin blood flow was measured before each injection. After 4 weeks of expansion, the sample was harvested for histological staining to measure the diameter and density of blood vessels; meanwhile, a 2 cm× 8 cm expanded random flap was elevated and sutured in situ. Blood flow perfusion and flap survival were observed.Results Compared with the control group, the BTX-A group had more blood flow, a larger blood vessel diameter, and higher blood vessel density in the expanded skin. Additionally, the flap of the BTX-A group had good blood flow perfusion and a high proportion of flap survival area within 7 days after expanded flap transfer. Data were analyzed using an independent t-test.Conclusion Pre-surgical BTX-A treatment may increase angiogenesis and vasodilatation, with subsequent blood perfusion elevation during and after expansion, and obtain a greater proportion of survival area of the transferred expanded flap.

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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Investigative Surgery publishes peer-reviewed scientific articles for the advancement of surgery, to the ultimate benefit of patient care and rehabilitation. It is the only journal that encompasses the individual and collaborative efforts of scientists in human and veterinary medicine, dentistry, basic and applied sciences, engineering, and law and ethics. The journal is dedicated to the publication of outstanding articles of interest to the surgical research community.
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