聚(2-乙烯基吡啶)作为正离子基质辅助激光解吸/电离-质谱法在不同仪器平台上的质量校准参考化合物。

IF 1.1 4区 化学 Q4 PHYSICS, ATOMIC, MOLECULAR & CHEMICAL
European Journal of Mass Spectrometry Pub Date : 2021-10-01 Epub Date: 2021-11-05 DOI:10.1177/14690667211055701
Jürgen H Gross
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引用次数: 0

摘要

对端丁基聚(2-乙烯基吡啶)(P2VP) C4H9(C7H7N)nH在正离子基质辅助激光解吸/电离质谱法(MALDI-MS)中用作外部和内部质量校准剂进行了评价。采用m/z 450-4500范围的P2VP低聚物对线性和反射模式下的飞行时间(TOF)质谱仪、离子迁移-四极飞行时间(IM-Q-TOF)质谱仪和傅里叶变换离子回旋共振(FT-ICR)质谱仪进行了校准。P2VPs的质子亲和性使得[M + H]+离子与常见的酸性基质,如α-氰基-4-羟基肉桂酸(CHCA)和2,5-二羟基苯甲酸(DHB),以及与非酸性和非质子基质1,8-二羟基- 10h -蒽-9-二蒽醇和2-[(2E)-3-(4-叔丁基苯基)-2-甲基丙基-2-苯基]丙腈(DCTB)几乎完全形成。在Δ(M /z) = 105.0578处均匀分布的[M + H]+离子的普遍存在使得端丁基P2VP低聚物成为方便的质量校准剂。通过使用已建立的标准化合物,使用不同的质量分析仪和操作模式,在各种m/z范围内实现的质量精度进行了评估。给出了内部和外部标定的结果。此外,还讨论了适合各自分析仪模式的质量参考清单的编制,并提供了这些参考文件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Poly(2-vinylpyridine) as a reference compound for mass calibration in positive-ion matrix-assisted laser desorption/ionization-mass spectrometry on different instrumental platforms.

Butyl-terminated poly(2-vinylpyridine) (P2VP), C4H9(C7H7N)nH, is evaluated for use as an external and internal mass calibrant in positive-ion matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). P2VP oligomers covering the m/z 450-4500 range are employed to calibrate a time-of-flight (TOF) mass spectrometer in linear and reflector mode, an ion mobility-quadrupole-time-of-flight (IM-Q-TOF) mass spectrometer, and a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. The proton affinity of P2VPs introduced by the numerous pyridyl groups leads to the almost exclusive formation of [M + H]+ ions with common acidic matrices like α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB) as well as with the non-acidic and aprotic matrices 1,8-dihydroxy-10H-anthracen-9-on (dithranol) and 2-[(2E)-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene]malonitrile (DCTB). This prevalence of [M + H]+ ions evenly spaced at Δ(m/z) = 105.0578 renders butyl-terminated P2VP oligomers as convenient mass calibrants. The mass accuracies achieved across various m/z ranges with different mass analyzers and modes of operation are evaluated by using established standard compounds. Results as obtained by internal or external calibration are presented. Further, the compilation of mass reference lists tailored to suit the respective analyzer modes is discussed and those reference files are provided.

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来源期刊
CiteScore
2.40
自引率
7.70%
发文量
16
审稿时长
>12 weeks
期刊介绍: JMS - European Journal of Mass Spectrometry, is a peer-reviewed journal, devoted to the publication of innovative research in mass spectrometry. Articles in the journal come from proteomics, metabolomics, petroleomics and other areas developing under the umbrella of the “omic revolution”.
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