Xintong Guo, Xiangying Ding, Qinpei Ding, Min Liang
{"title":"地塞米松诱导成骨细胞凋亡的作用具有持续时间和剂量依赖性。","authors":"Xintong Guo, Xiangying Ding, Qinpei Ding, Min Liang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Osteoblasts play an important role in the process of osteogenesis and prevention of osteonecrosis. Dexamethasone, a type of glucocorticoids (GCs), induce apoptosis of osteoblasts and lead to the occurrence of non-traumatic osteonecrosis. This study aimed to explore the effects of different doses and duration of Dexamethasone on osteoblast apoptosis of rats in vitro.</p><p><strong>Methods: </strong>Proliferation and apoptosis of osteoblasts after Dexamethasone treatment were detected using cell counting kit-8 (CCK-8) assay and FITC-Annexin V/PI staining. The expressions of caspase-3 and -9 in osteoblasts after Dexamethasone treatment were analyzed using western blotting and qRT-PCR. Dexamethasone remarkably inhibited proliferation and induced apoptosis of osteoblasts in a dose-and duration-dependent manner.</p><p><strong>Results: </strong>As the intervention time extended, the expression of caspase-3 mRNA and caspase-9 mRNA in different Dexamethasone groups gradually increased in a duration-dependent manner. With the same time of intervention (12h, 24h, 48h), the expression of caspase-3 and -9 mRNA gradually increased in a dose-dependent manner. After treated with 5 * 10-8M, 5 * 10-7M, 5 * 10-6M and 5 * 10-5M Dexamethasone for 24 hours, the expression of cleaved caspase-3 and -9 protein increased in a dose-dependent manner.</p><p><strong>Conclusion: </strong>Dexamethasone can induce osteoblast apoptosis in a duration- and dose-dependent manner.</p>","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dexamethasone induce osteoblast apoptosis in a duration- and dose-dependent manner.\",\"authors\":\"Xintong Guo, Xiangying Ding, Qinpei Ding, Min Liang\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Osteoblasts play an important role in the process of osteogenesis and prevention of osteonecrosis. Dexamethasone, a type of glucocorticoids (GCs), induce apoptosis of osteoblasts and lead to the occurrence of non-traumatic osteonecrosis. This study aimed to explore the effects of different doses and duration of Dexamethasone on osteoblast apoptosis of rats in vitro.</p><p><strong>Methods: </strong>Proliferation and apoptosis of osteoblasts after Dexamethasone treatment were detected using cell counting kit-8 (CCK-8) assay and FITC-Annexin V/PI staining. The expressions of caspase-3 and -9 in osteoblasts after Dexamethasone treatment were analyzed using western blotting and qRT-PCR. Dexamethasone remarkably inhibited proliferation and induced apoptosis of osteoblasts in a dose-and duration-dependent manner.</p><p><strong>Results: </strong>As the intervention time extended, the expression of caspase-3 mRNA and caspase-9 mRNA in different Dexamethasone groups gradually increased in a duration-dependent manner. With the same time of intervention (12h, 24h, 48h), the expression of caspase-3 and -9 mRNA gradually increased in a dose-dependent manner. After treated with 5 * 10-8M, 5 * 10-7M, 5 * 10-6M and 5 * 10-5M Dexamethasone for 24 hours, the expression of cleaved caspase-3 and -9 protein increased in a dose-dependent manner.</p><p><strong>Conclusion: </strong>Dexamethasone can induce osteoblast apoptosis in a duration- and dose-dependent manner.</p>\",\"PeriodicalId\":0,\"journal\":{\"name\":\"\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0,\"publicationDate\":\"2021-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Dexamethasone induce osteoblast apoptosis in a duration- and dose-dependent manner.
Objectives: Osteoblasts play an important role in the process of osteogenesis and prevention of osteonecrosis. Dexamethasone, a type of glucocorticoids (GCs), induce apoptosis of osteoblasts and lead to the occurrence of non-traumatic osteonecrosis. This study aimed to explore the effects of different doses and duration of Dexamethasone on osteoblast apoptosis of rats in vitro.
Methods: Proliferation and apoptosis of osteoblasts after Dexamethasone treatment were detected using cell counting kit-8 (CCK-8) assay and FITC-Annexin V/PI staining. The expressions of caspase-3 and -9 in osteoblasts after Dexamethasone treatment were analyzed using western blotting and qRT-PCR. Dexamethasone remarkably inhibited proliferation and induced apoptosis of osteoblasts in a dose-and duration-dependent manner.
Results: As the intervention time extended, the expression of caspase-3 mRNA and caspase-9 mRNA in different Dexamethasone groups gradually increased in a duration-dependent manner. With the same time of intervention (12h, 24h, 48h), the expression of caspase-3 and -9 mRNA gradually increased in a dose-dependent manner. After treated with 5 * 10-8M, 5 * 10-7M, 5 * 10-6M and 5 * 10-5M Dexamethasone for 24 hours, the expression of cleaved caspase-3 and -9 protein increased in a dose-dependent manner.
Conclusion: Dexamethasone can induce osteoblast apoptosis in a duration- and dose-dependent manner.
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