{"title":"马源弯曲杆菌分离株部分毒力基因的监测","authors":"Marek Selwet","doi":"10.18388/pb.2021_384","DOIUrl":null,"url":null,"abstract":"<p><p>The research concerned the determination of the frequency of occurrence of selected virulence genes (cadF, flaA, iam) and genes responsible for the formation of the CDT cytotoxin (cdtA, cdtB, cdtC) in Campylobacter spp. The research object consisted of 100 faecal samples collected from stallions showing no symptoms of campylobacteriosis. The presence of bacteria of the genus Campylobacter spp. Was found in 25 individuals (25%). The molecular biology techniques used in the research allowed us to distinguish the following species from the positive samples: C. jejuni (68%); C. coli (28%) and C. lari (4%). In total, the following genes were found within the marked species: cadF (n=10); flaA (n=5); iam (n=3); cdtA (n=1); cdtB (n=10) and cdtC (n=2). In none of the obtained isolates, the simultaneous presence of genes responsible for the synthesis of CDT toxin was found.</p>","PeriodicalId":20335,"journal":{"name":"Postepy biochemii","volume":"67 2","pages":"172-176"},"PeriodicalIF":0.0000,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Monitoring of selected virulence genes in Campylobacter spp. isolates obtained from horses\",\"authors\":\"Marek Selwet\",\"doi\":\"10.18388/pb.2021_384\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The research concerned the determination of the frequency of occurrence of selected virulence genes (cadF, flaA, iam) and genes responsible for the formation of the CDT cytotoxin (cdtA, cdtB, cdtC) in Campylobacter spp. The research object consisted of 100 faecal samples collected from stallions showing no symptoms of campylobacteriosis. The presence of bacteria of the genus Campylobacter spp. Was found in 25 individuals (25%). The molecular biology techniques used in the research allowed us to distinguish the following species from the positive samples: C. jejuni (68%); C. coli (28%) and C. lari (4%). In total, the following genes were found within the marked species: cadF (n=10); flaA (n=5); iam (n=3); cdtA (n=1); cdtB (n=10) and cdtC (n=2). In none of the obtained isolates, the simultaneous presence of genes responsible for the synthesis of CDT toxin was found.</p>\",\"PeriodicalId\":20335,\"journal\":{\"name\":\"Postepy biochemii\",\"volume\":\"67 2\",\"pages\":\"172-176\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Postepy biochemii\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18388/pb.2021_384\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/6/30 0:00:00\",\"PubModel\":\"Print\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Postepy biochemii","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18388/pb.2021_384","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/6/30 0:00:00","PubModel":"Print","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Monitoring of selected virulence genes in Campylobacter spp. isolates obtained from horses
The research concerned the determination of the frequency of occurrence of selected virulence genes (cadF, flaA, iam) and genes responsible for the formation of the CDT cytotoxin (cdtA, cdtB, cdtC) in Campylobacter spp. The research object consisted of 100 faecal samples collected from stallions showing no symptoms of campylobacteriosis. The presence of bacteria of the genus Campylobacter spp. Was found in 25 individuals (25%). The molecular biology techniques used in the research allowed us to distinguish the following species from the positive samples: C. jejuni (68%); C. coli (28%) and C. lari (4%). In total, the following genes were found within the marked species: cadF (n=10); flaA (n=5); iam (n=3); cdtA (n=1); cdtB (n=10) and cdtC (n=2). In none of the obtained isolates, the simultaneous presence of genes responsible for the synthesis of CDT toxin was found.
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