紧密连接相关的MARVEL蛋白marvelD3在肝癌迁移和上皮间质转化中的作用。

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Yanmeng Li, Teng Li, Donghu Zhou, Jia Wei, Zhenkun Li, Xiaojin Li, Siyu Jia, Qin Ouyang, Saiping Qi, Zhibin Chen, Bei Zhang, Jing Yu, Jidong Jia, Anjian Xu, Jian Huang
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引用次数: 7

摘要

最近发现的紧密连接膜蛋白MarvelD3可能与肝细胞癌(HCC)有关。我们旨在研究marvelD3在HCC上皮-间质转化(Epithelial-Mesenchymal Transition, EMT)和迁移中的作用,并探讨其潜在的分子机制。首先,我们评估了HCC和正常肝组织中marvelD3的表达,发现marvelD3表达缺失与HCC的发生和TNM分期显著相关。其次,我们检测到转化生长因子β1和蜗牛/鼻涕虫诱导的EMT在HCC细胞中下调了marvelD3。最后,我们分析了marvelD3蛋白的表达与EMT和NF-κB信号通路显著相关。我们的研究表明,MarvelD3通过抑制NF-κB信号通路抑制HCC细胞的EMT和迁移。缩写:HCC,肝细胞癌;TJ,紧密连接;用于囊泡运输和膜连接的MARVEL、MAL及相关蛋白;上皮-间质转化;NF-κB,核因子κB;TAMPs,紧密连接相关的奇迹蛋白;TGF-β1,转化生长因子-β1;基质金属肽酶9;RT-PCR, Real-time PCR;包含IHC,免疫组织化学;如果,免疫荧光。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Role of tight junction-associated MARVEL protein marvelD3 in migration and epithelial-mesenchymal transition of hepatocellular carcinoma.

Role of tight junction-associated MARVEL protein marvelD3 in migration and epithelial-mesenchymal transition of hepatocellular carcinoma.

Role of tight junction-associated MARVEL protein marvelD3 in migration and epithelial-mesenchymal transition of hepatocellular carcinoma.

Role of tight junction-associated MARVEL protein marvelD3 in migration and epithelial-mesenchymal transition of hepatocellular carcinoma.

MarvelD3, a recently identified tight junction membrane protein, could be associated with hepatocellular carcinoma (HCC). We aimed to investigate the role of marvelD3 in Epithelial-Mesenchymal Transition (EMT) and migration of HCC and explore the underlying molecular mechanisms. First, we assessed marvlD3 expression in HCC and normal liver tissues and found loss of marvelD3 expression was significantly correlated with the occurrence and TNM stage of HCC. Second, we detected that marvelD3 was downregulated in HCC cells with transforming growth factor β1 and snail/slug-induced EMT. Finally, we analyzed expression of marvelD3 protein was significantly associated with EMT and the NF-κB signaling pathway. Our study demonstrated that MarvelD3 inhibited EMT and migration of HCC cells along with inhibiting NF-κB signaling pathway.Abbreviations:HCC, Hepatocellular carcinoma; TJ, Tight junction; MARVEL, MAL and related proteins for vesicle trafficking and membrane link; EMT, Epithelial-mesenchymal transition; NF-κB, Nuclear factor kappa B; TAMPs, Tight junction-associated marvel proteins; TGF-β1, Transforming growth factor-β1; MMP9, matrix metallopeptidase 9; RT-PCR, Real-time PCR; IHC, Immunohistochemistry; IF, Immunofluorescence.

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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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