在处理前将染料应用于细胞学细胞块和活检组织，可以增强包埋和显微切开术期间标本的可视化。

IF 0.6 4区生物学 Q4 CELL BIOLOGY

Journal of Histotechnology Pub Date : 2021-12-01 Epub Date: 2021-06-16 DOI:10.1080/01478885.2021.1909357

Sheila Criswell, Jada Sutton

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引用次数: 1

摘要

细胞学标本和活检组织经常小而苍白，使它们难以在石蜡中肉眼可见。在小组织上检测了10种染料，以确定在组织过程中的包埋和显微切片步骤中是否可以增加标本的可辨别性。理想的染料在随后的染色后在组织切片显微镜下不能保持可见，并且不能干扰免疫组织化学(IHC)检测。本研究发现Harris苏木精和1%甲苯胺蓝溶液是大体组织可视化的最佳标记剂，并且对后处理染色和免疫组化检测没有不利影响。

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Application of dyes to cytology cell blocks and biopsy tissues before processing enhances specimen visualization during embedding and microtomy.

Cytology specimens and biopsy tissues are frequently small and pale, making them difficult to visualize grossly in paraffin. Ten dyes were assayed on small tissues to determine if specimen discernibility could be increased during the embedding and microtomy steps in the histological process. The ideal dye should not remain visible in a tissue section microscopically after subsequent staining and must not interfere with immunohistochemistry (IHC) assays. This study found that Harris hematoxylin and 1% aq. toluidine blue solution were the best labelers for gross tissue visualization and did not adversely affect post-processing staining and IHC assays.

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来源期刊

Journal of Histotechnology 生物-细胞生物学

CiteScore

2.60

自引率

9.10%

发文量

审稿时长

>12 weeks

期刊介绍： The official journal of the National Society for Histotechnology, Journal of Histotechnology, aims to advance the understanding of complex biological systems and improve patient care by applying histotechniques to diagnose, prevent and treat diseases. Journal of Histotechnology is concerned with educating practitioners and researchers from diverse disciplines about the methods used to prepare tissues and cell types, from all species, for microscopic examination. This is especially relevant to Histotechnicians. Journal of Histotechnology welcomes research addressing new, improved, or traditional techniques for tissue and cell preparation. This includes review articles, original articles, technical notes, case studies, advances in technology, and letters to editors. Topics may include, but are not limited to, discussion of clinical, veterinary, and research histopathology.