Alexon M Racilan, Wiviane A Assis, Maíra Casalechi, Ananda Spagnolo-Souza, Marcelo A Pascoal-Xavier, Ana C Simões-E-Silva, Helen L Del Puerto, Fernando M Reis
{"title":"血管紧张素转换酶2是SARS-CoV-2细胞受体，在人子宫肌层和子宫平滑肌瘤中广泛表达。","authors":"Alexon M Racilan, Wiviane A Assis, Maíra Casalechi, Ananda Spagnolo-Souza, Marcelo A Pascoal-Xavier, Ana C Simões-E-Silva, Helen L Del Puerto, Fernando M Reis","doi":"10.1177/2284026520954068","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Angiotensin-converting-enzyme 2 (ACE2), the cell surface receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is found in a variety of reproductive tissues. The present study evaluated whether uterine fibroids and normal myometrium express ACE2 and, if so, at which tissue compartments.</p><p><strong>Methods: </strong>We included 13 premenopausal women (age range 33-50 years, median 40 years) with uterine fibroids undergoing elective hysterectomy or myomectomy. Samples of leiomyoma (<i>n</i> = 12) and normal myometrial tissue (<i>n</i> = 8) were analyzed by immunohistochemistry for protein localization or by real time PCR for mRNA detection.</p><p><strong>Results: </strong>In normal myometrium, ACE2 immunoreactivity was localized in smooth muscle fibers, arteriolar walls, and endothelial cells. In uterine leiomyoma, ACE2 staining was more intense in smooth muscle cells than in the extracellular matrix, and was also present in vascular endothelium. ACE2 mRNA was detected in myometrium as well as in fibroid samples.</p><p><strong>Conclusion: </strong>Human myometrium and uterine leiomyoma express ACE2 mRNA and have abundant distribution of ACE2 protein in their smooth muscle cells and microvasculature.</p>","PeriodicalId":15725,"journal":{"name":"Journal of endometriosis and pelvic pain disorders","volume":"13 1","pages":"20-24"},"PeriodicalIF":0.6000,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/2284026520954068","citationCount":"4","resultStr":"{\"title\":\"Angiotensin-converting enzyme 2, the SARS-CoV-2 cellular receptor, is widely expressed in human myometrium and uterine leiomyoma.\",\"authors\":\"Alexon M Racilan, Wiviane A Assis, Maíra Casalechi, Ananda Spagnolo-Souza, Marcelo A Pascoal-Xavier, Ana C Simões-E-Silva, Helen L Del Puerto, Fernando M Reis\",\"doi\":\"10.1177/2284026520954068\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Angiotensin-converting-enzyme 2 (ACE2), the cell surface receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is found in a variety of reproductive tissues. The present study evaluated whether uterine fibroids and normal myometrium express ACE2 and, if so, at which tissue compartments.</p><p><strong>Methods: </strong>We included 13 premenopausal women (age range 33-50 years, median 40 years) with uterine fibroids undergoing elective hysterectomy or myomectomy. Samples of leiomyoma (<i>n</i> = 12) and normal myometrial tissue (<i>n</i> = 8) were analyzed by immunohistochemistry for protein localization or by real time PCR for mRNA detection.</p><p><strong>Results: </strong>In normal myometrium, ACE2 immunoreactivity was localized in smooth muscle fibers, arteriolar walls, and endothelial cells. In uterine leiomyoma, ACE2 staining was more intense in smooth muscle cells than in the extracellular matrix, and was also present in vascular endothelium. ACE2 mRNA was detected in myometrium as well as in fibroid samples.</p><p><strong>Conclusion: </strong>Human myometrium and uterine leiomyoma express ACE2 mRNA and have abundant distribution of ACE2 protein in their smooth muscle cells and microvasculature.</p>\",\"PeriodicalId\":15725,\"journal\":{\"name\":\"Journal of endometriosis and pelvic pain disorders\",\"volume\":\"13 1\",\"pages\":\"20-24\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2021-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1177/2284026520954068\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of endometriosis and pelvic pain disorders\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1177/2284026520954068\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of endometriosis and pelvic pain disorders","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/2284026520954068","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
Angiotensin-converting enzyme 2, the SARS-CoV-2 cellular receptor, is widely expressed in human myometrium and uterine leiomyoma.
Objective: Angiotensin-converting-enzyme 2 (ACE2), the cell surface receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is found in a variety of reproductive tissues. The present study evaluated whether uterine fibroids and normal myometrium express ACE2 and, if so, at which tissue compartments.
Methods: We included 13 premenopausal women (age range 33-50 years, median 40 years) with uterine fibroids undergoing elective hysterectomy or myomectomy. Samples of leiomyoma (n = 12) and normal myometrial tissue (n = 8) were analyzed by immunohistochemistry for protein localization or by real time PCR for mRNA detection.
Results: In normal myometrium, ACE2 immunoreactivity was localized in smooth muscle fibers, arteriolar walls, and endothelial cells. In uterine leiomyoma, ACE2 staining was more intense in smooth muscle cells than in the extracellular matrix, and was also present in vascular endothelium. ACE2 mRNA was detected in myometrium as well as in fibroid samples.
Conclusion: Human myometrium and uterine leiomyoma express ACE2 mRNA and have abundant distribution of ACE2 protein in their smooth muscle cells and microvasculature.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
