干扰素治疗后肝癌复发相关mirna、mrna、lncrna和circrna的鉴定

IF 0.8 4区 医学 Q4 ENDOCRINOLOGY & METABOLISM
W P Zhu, X G He, H X Zhu, L R Wang, Z H Lin, M Wang, L Wang
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引用次数: 1

摘要

为了研究干扰素-α (IFN-α)在肝细胞癌(HCC)治疗中的分子机制及预测疗效的分子标志物,我们从4例未接受治疗的患者(对照组)、4例接受重组人干扰素a-2b治疗的患者(case1)中收集12份样本,筛选差异表达(DE)-miRNAs、- mrna、-lncRNAs和-circRNAs。接受重组人干扰素a-2b治疗后复发4例(case2)。进行富集分析以确定de - rna的主要功能。我们还构建了蛋白质-蛋白质相互作用(PPI)和竞争内源性RNA (ceRNA)网络。此外,我们还进行了系列聚类分析,以分析不同HCC组中基因表达的变化。此外,这些基因的表达在癌症基因组图谱(TCGA)数据库中得到验证。在对照组与case1组、case2组与case1组之间共获得36个联合de - mirna、175个联合de - mrna、65个联合de - lncrna和52个联合de - circrna。de - mrna主要参与线粒体内膜。de - circrna主要富集在高尔基体中。ceRNA网络包含68个de - mrna, 26个de - mirna, 45个de - lncrna和23个de - circrna。共有24个de - mirna、175个de - mrna、65个de - lncrna和52个de - circrna分别被划分为8个谱。26个基因与HCC预后有显著相关性(p < 0.05)。一些基因可用于预测IFN-α治疗HCC的疗效。研究结果可为探讨IFN-α在HCC治疗中的不同敏感性奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of miRNAs, mRNAs, lncRNAs, and circRNAs associated with hepatocellular carcinoma recurrence after interferon treatment.

To study the molecular mechanism of interferon-alpha (IFN-α) in the treatment of hepatocellular carcinoma (HCC) and the molecular markers that can predict the therapeutic effect, differentially expressed (DE)-miRNAs, -mRNAs, -lncRNAs, and -circRNAs were screened between 12 samples collected from 4 patients who had not received treatment (control), 4 patients who had received recombinant human interferon a-2b treatment (case1), and 4 patients who had relapsed after receiving recombinant human interferon a-2b treatment (case2). Enrichment analyses were performed to determine the principal functions of the DE-RNAs. We also constructed protein-protein interactions (PPI) and competing endogenous RNA (ceRNA) networks. In addition, a series-cluster analysis was performed to analyze changes in gene expression across different groups of HCC. Furthermore, the expression of the genes were verified in the Cancer Genome Atlas (TCGA) database. A total of 36 union DE-miRNAs, 175 union DE-mRNAs, 65 union DE-lncRNAs, and 52 union DE-circRNAs were obtained between the control vs case1, and case2 vs case1 groups. DE-mRNAs were mainly involved in the mitochondrial inner membrane. DE-circRNAs were mainly enriched in the Golgi apparatus. ceRNA network contained 68 DE-mRNAs, 26 DE-miRNAs, 45 DE-lncRNAs, and 23 DE-circRNAs. A total of 24 DE-miRNAs, 175 DE-mRNAs, 65 DE-lncRNAs, and 52 DE-circRNAs were classified into eight profiles, respectively. A total of 26 genes showed a significant correlation with prognosis of HCC (p < 0.05). Some genes may be used to predict the efficacy of IFN-α in the treatment of HCC. The results may lay a foundation for investigating the different sensitivities of IFN-α in the treatment of HCC.

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来源期刊
CiteScore
2.20
自引率
15.60%
发文量
0
审稿时长
6 months
期刊介绍: Journal of Biological Regulators & Homeostatic Agents (IF 1.397) is a peer-reviewed journal published every 2 months. The journal publishes original papers describing research in the fields of experimental and clinical medicine, molecular biology, biochemistry, regulatory molecules, cellular immunology and pharmacology.
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