人类射精中携带y染色体精子含量百分比的个体差异。

IF 2.1 4区医学 Q3 ANDROLOGY

Systems Biology in Reproductive Medicine Pub Date : 2021-10-01 Epub Date: 2021-07-12 DOI:10.1080/19396368.2021.1942589

Rajasingam S Jeyendran, Jared Graham, Saranya Tharma, Milica Ivanovic, Seth Levrant, Hakan M Ozornek, Morry B Fiddler

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引用次数: 0

摘要

该研究旨在确定个体射精中携带y染色体的精子含量的变化。一种实时聚合酶链反应(qPCR)与独特的引物开发，并用于计算携带y染色体精子的百分比从50随机选择的男性个体射精。携带y染色体的精子比例与携带x染色体的精子比例的总体平均值±SD差异有统计学意义(45.36±7.88∶54.42±7.88)。在50例射精中，17例大于y染色体精子平均值的99%置信区间(45.58±2.87)，14例小于y染色体精子平均值的99%置信区间(45.58±2.87)。这些结果表明，基于精子的性别选择结果的不一致似乎是射精差异的一个功能，并强调需要进一步研究环境和遗传因素对X或Y生殖精子不稳定性的影响。缩写:qPCR:实时聚合酶链反应;ROS:活性氧;德勤:二硫苏糖醇;SRY:性别决定区域Y。

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Individual variation of the percentage of Y-chromosome bearing sperm content in human ejaculates.

The study aimed to determine the variation of Y-chromosome-bearing sperm content among individual ejaculates. A real-time polymerase chain reaction (qPCR) with unique primers was developed and used to calculate the percentage of Y-chromosome-bearing sperm in individual ejaculates from 50 randomly selected men. There was a significant difference in the overall mean ± SD between the proportion of Y-chromosome-bearing sperm and X-chromosome-bearing sperm (45.36 ± 7.88 vs. 54.42 ± 7.88). Of the 50 ejaculates, 17 had more than, and 14 had less than the 99% confidence interval of the mean of the Y-chromosome-bearing sperm (45.58 ± 2.87). These results suggest that the inconsistency in sperm-based sex-selection outcomes appears to be a function of differences in the ejaculates and highlights the need for further study in environmental and genetic factors contributing to X or Y bearing spermatozoan instability.Abbreviations: qPCR: real-time polymerase chain reaction; ROS: reactive oxygen species; DTT: dithiothreitol; SRY: sex-determining region Y.

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来源期刊

Systems Biology in Reproductive Medicine 医学-男科学

CiteScore

4.30

自引率

4.20%

发文量

审稿时长

>12 weeks

期刊介绍： Systems Biology in Reproductive Medicine, SBiRM, publishes Research Articles, Communications, Applications Notes that include protocols a Clinical Corner that includes case reports, Review Articles and Hypotheses and Letters to the Editor on human and animal reproduction. The journal will highlight the use of systems approaches including genomic, cellular, proteomic, metabolomic, bioinformatic, molecular, and biochemical, to address fundamental questions in reproductive biology, reproductive medicine, and translational research. The journal publishes research involving human and animal gametes, stem cells, developmental biology and toxicology, and clinical care in reproductive medicine. Specific areas of interest to the journal include: male factor infertility and germ cell biology, reproductive technologies (gamete micro-manipulation and cryopreservation, in vitro fertilization/embryo transfer (IVF/ET) and contraception. Research that is directed towards developing new or enhanced technologies for clinical medicine or scientific research in reproduction is of significant interest to the journal.