右美托咪定纳米金调控GSK-3β减轻麻醉大鼠神经认知效应

Journal of nanoscience and nanotechnology Pub Date : 2021-12-01 DOI:10.1166/jnn.2021.18745

Xiaoxia Zhang, Zumin Xing, Jiyuan Li, Shuyi Tang, Yiwen Zhang

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引用次数: 0

摘要

本研究旨在探讨右美托咪定负载金纳米颗粒(aunps -右美托咪定)对麻醉大鼠神经认知的影响。Sprague Dawley大鼠60只(2-3周龄);体重250 ~ 280 g)，随机分为3组(n = 20):对照组和2组分别腹腔注射aunps -右美托咪定50和100 μg/kg。采用Western blotting和RT-PCR分别检测GSK-3β蛋白和mRNA的表达。与对照组相比，aunp -右美托咪定组GSK-3β表达升高(P < 0.05)。100 μg/kg aunp -右美托咪定组GSK-3β蛋白表达量显著升高，mRNA表达量显著降低(P < 0.05)。aunps -右美托咪定通过调节GSK-3β信号通路降低麻醉大鼠的神经认知作用。

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Gold Nanoparticles with Dexmedetomidine Regulate GSK-3β to Reduce Neurocognitive Effects in Anesthetized Rats.

The aim of this study was to explore the neurocognitive effects of dexmedetomidine-loaded gold nanoparticles (AuNPs-dexmedetomidine) on anesthetized rats. Sixty Sprague Dawley rats (age, 2-3 weeks; weight, 250-280 g) were randomly divided into three groups (n = 20): the control group and two groups that received intraperitoneal injection of AuNPs-dexmedetomidine at 50 and 100 μg/kg each. Western blotting and RT-PCR were used to determine the protein and mRNA expression of GSK-3β, respectively. Compared with that in the control group, GSK-3β expression in AuNP-dexmedetomidine groups increased (P < 0.05). The protein expression of GSK-3β was higher and mRNA expression was significantly lower in the 100 μg/kg AuNP-dexmedetomidine group (P < 0.05). AuNPs-dexmedetomidine reduced the neurocognitive effect on anesthetized rats through the regulation of the GSK-3β signaling pathway.

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来源期刊

Journal of nanoscience and nanotechnology 工程技术-材料科学：综合

自引率

0.00%

发文量

审稿时长

3.6 months

期刊介绍： JNN is a multidisciplinary peer-reviewed journal covering fundamental and applied research in all disciplines of science, engineering and medicine. JNN publishes all aspects of nanoscale science and technology dealing with materials synthesis, processing, nanofabrication, nanoprobes, spectroscopy, properties, biological systems, nanostructures, theory and computation, nanoelectronics, nano-optics, nano-mechanics, nanodevices, nanobiotechnology, nanomedicine, nanotoxicology.