人类脂肪细胞在肾上腺素能刺激下的高效生热反应需要依赖 ASC-1 转运体的氨基酸摄取。

IF 3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
FEBS Letters Pub Date : 2021-08-01 Epub Date: 2021-07-23 DOI:10.1002/1873-3468.14155
Rini Arianti, Boglárka Ágnes Vinnai, Beáta B Tóth, Abhirup Shaw, Éva Csősz, Attila Vámos, Ferenc Győry, Pamela Fischer-Posovszky, Martin Wabitsch, Endre Kristóf, László Fésüs
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引用次数: 0

摘要

棕色和米色脂肪细胞通过依赖性解偶联蛋白1(UCP1)和非依赖性解偶联蛋白1(UCP1)产热来耗散能量,这可能被用来开发肥胖症的治疗方法。我们发现,丙氨酸/丝氨酸/半胱氨酸转运体-1(ASC-1)的mRNA和蛋白表达在人棕色易感深颈和米色易感皮下颈原细胞以及SGBS前脂肪细胞的脂肪细胞分化过程中被诱导。cAMP 刺激分化的脂肪细胞会导致丝氨酸、半胱氨酸和甘氨酸的摄取量增加,同时耗氧量增加,UCP1 依赖性质子泄漏增加,肌酸驱动的底物循环耦合呼吸增加,产热标记基因和几个呼吸复合体亚基上调;这些结果在特异性 ASC-1 抑制剂 BMS-466442 的存在下受到阻碍。我们的数据表明,ASC-1对丝氨酸、半胱氨酸和甘氨酸的依赖性消耗是有效刺激人类脂肪细胞产热所必需的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ASC-1 transporter-dependent amino acid uptake is required for the efficient thermogenic response of human adipocytes to adrenergic stimulation.

Brown and beige adipocytes dissipate energy by uncoupling protein 1 (UCP1)-dependent and UCP1-independent thermogenesis, which may be utilized to develop treatments against obesity. We have found that mRNA and protein expression of the alanine/serine/cysteine transporter-1 (ASC-1) was induced during adipocyte differentiation of human brown-prone deep neck and beige-competent subcutaneous neck progenitors, and SGBS preadipocytes. cAMP stimulation of differentiated adipocytes led to elevated uptake of serine, cysteine, and glycine, in parallel with increased oxygen consumption, augmented UCP1-dependent proton leak, increased creatine-driven substrate cycle-coupled respiration, and upregulation of thermogenesis marker genes and several respiratory complex subunits; these outcomes were impeded in the presence of the specific ASC-1 inhibitor, BMS-466442. Our data suggest that ASC-1-dependent consumption of serine, cysteine, and glycine is required for efficient thermogenic stimulation of human adipocytes.

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来源期刊
FEBS Letters
FEBS Letters 生物-生化与分子生物学
CiteScore
6.60
自引率
2.90%
发文量
303
审稿时长
1 months
期刊介绍: FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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