褪黑素对创伤性脑损伤大鼠皮质神经元再生的影响。

IF 1.2 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Clinical and Investigative Medicine Pub Date : 2020-12-27 DOI:10.25011/cim.v43i4.34829

Jianbin Ge, Dandan Chen, Jingjing Ben, Xinjian Song, Linqing Zou, Xin Yi

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引用次数: 3

摘要

目的:探讨褪黑素对创伤性脑损伤(TBI)大鼠皮质神经元再生的影响。方法:36只sd - dawley大鼠随机分为假手术组、TBI+载药组和TBI+褪黑素组。分别用激光多普勒血流仪和Morris水迷宫法观察脑血流和认知功能。用血清丙二醛(MDA)和超氧化物歧化酶(SOD)水平评估氧化应激。采用免疫荧光法和末端脱氧核苷酸转移酶dUTP缺口末端标记法观察新生神经元和凋亡细胞。结果:损伤后1、12、24、48 h, TBI+褪黑素组脑血流量明显高于TBI+载药组，但差异无统计学意义(P>0.05)。TBI+褪黑素组大鼠认知功能明显优于TBI+载药组(P。

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Effect of melatonin on regeneration of cortical neurons in rats with traumatic brain injury.

Purpose: To investigate the effect of melatonin on regeneration of cortical neurons in rats with traumatic brain injury (TBI).

Methods: Sprague-Dawley rats (n=36) were randomly divided into sham, TBI+vehicle and TBI+melatonin groups. Cerebral blood flow and cognitive function were observed via laser Doppler flowmetry and by Morris water maze testing, respectively. The serum malondialdehyde (MDA) and superoxide dismutase (SOD) levels were used to assess oxidative stress. Immunofluorescence and terminal deoxynucleotidyl transferase dUTP nick end labelling assay was used to observe the newborn neurons and apoptotic cells.

Results: Cerebral blood flow in the TBI+melatonin group was higher than that of the TBI+vehicle group at one, 12, 24 and 48 h post-injury, but the difference was not statistically significant (P>0.05). The cognitive function of the rats was better in the TBI+melatonin group than the TBI+vehicle group (P.

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来源期刊

Clinical and Investigative Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

12.50%

发文量

审稿时长

>12 weeks

期刊介绍： Clinical and Investigative Medicine (CIM), publishes original work in the field of Clinical Investigation. Original work includes clinical or laboratory investigations and clinical reports. Reviews include information for Continuing Medical Education (CME), narrative review articles, systematic reviews, and meta-analyses.