{"title":"R-spondins/Lgrs在颅面活动器官发育中的表达","authors":"Jun Nihara , Maiko Kawasaki , Katsushige Kawasaki , Akane Yamada , Fumiya Meguro , Takehisa Kudo , Supaluk Trakanant , Takahiro Nagai , Isao Saito , Takeyasu Maeda , Atsushi Ohazama","doi":"10.1016/j.gep.2021.119195","DOIUrl":null,"url":null,"abstract":"<div><p>Wnt signaling plays a critical role in the development of many organs, including the major movable craniofacial organs tongue, lip, and eyelid. Four members of the R-spondin family (<em>Rspo1</em>–<em>4</em>) bind to <em>Lgr4</em>/<em>5</em>/<em>6</em> to regulate the activation of Wnt signaling. However, it is not fully understood how <em>Rspos</em>/<em>Lgrs</em> regulate Wnt signaling during the development of movable craniofacial organs. To address this question, we examined the expression of <em>Rspos</em>, <em>Lgrs</em>, and <em>Axin2</em> (major mediator of canonical Wnt signaling) during tongue, lip, and eyelid development. The expression of <em>Axin2</em>, <em>Rspos</em> and <em>Lgrs</em> was observed in many similar regions, suggesting that <em>Rspos</em> likely activate canonical Wnt signaling through the <em>Lgr</em>-dependent pathway in these regions. <em>Lgr</em> expression was not detected in regions where <em>Axin2</em> and <em>Rspos</em> were expressed, suggesting that <em>Rspos</em> might activate canonical Wnt signaling through the <em>Lgr</em>-independent pathway in these regions. In addition, the expression of <em>Rspos</em> and <em>Lgrs</em> were observed in some other regions where <em>Axin2</em> was not expressed, suggesting the possibility that <em>Rspos</em> and/or <em>Lgrs</em> are involved in non-canonical Wnt signaling or the Wnt-independent pathway. Thus, we identified a dynamic spatiotemporal expression pattern of <em>Rspos</em> and <em>Lgrs</em> during the development of the eyelid, tongue, and lip.</p></div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gep.2021.119195","citationCount":"2","resultStr":"{\"title\":\"Expression of R-spondins/Lgrs in development of movable craniofacial organs\",\"authors\":\"Jun Nihara , Maiko Kawasaki , Katsushige Kawasaki , Akane Yamada , Fumiya Meguro , Takehisa Kudo , Supaluk Trakanant , Takahiro Nagai , Isao Saito , Takeyasu Maeda , Atsushi Ohazama\",\"doi\":\"10.1016/j.gep.2021.119195\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Wnt signaling plays a critical role in the development of many organs, including the major movable craniofacial organs tongue, lip, and eyelid. Four members of the R-spondin family (<em>Rspo1</em>–<em>4</em>) bind to <em>Lgr4</em>/<em>5</em>/<em>6</em> to regulate the activation of Wnt signaling. However, it is not fully understood how <em>Rspos</em>/<em>Lgrs</em> regulate Wnt signaling during the development of movable craniofacial organs. To address this question, we examined the expression of <em>Rspos</em>, <em>Lgrs</em>, and <em>Axin2</em> (major mediator of canonical Wnt signaling) during tongue, lip, and eyelid development. The expression of <em>Axin2</em>, <em>Rspos</em> and <em>Lgrs</em> was observed in many similar regions, suggesting that <em>Rspos</em> likely activate canonical Wnt signaling through the <em>Lgr</em>-dependent pathway in these regions. <em>Lgr</em> expression was not detected in regions where <em>Axin2</em> and <em>Rspos</em> were expressed, suggesting that <em>Rspos</em> might activate canonical Wnt signaling through the <em>Lgr</em>-independent pathway in these regions. In addition, the expression of <em>Rspos</em> and <em>Lgrs</em> were observed in some other regions where <em>Axin2</em> was not expressed, suggesting the possibility that <em>Rspos</em> and/or <em>Lgrs</em> are involved in non-canonical Wnt signaling or the Wnt-independent pathway. Thus, we identified a dynamic spatiotemporal expression pattern of <em>Rspos</em> and <em>Lgrs</em> during the development of the eyelid, tongue, and lip.</p></div>\",\"PeriodicalId\":55598,\"journal\":{\"name\":\"Gene Expression Patterns\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2021-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.gep.2021.119195\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene Expression Patterns\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567133X21000302\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Expression Patterns","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567133X21000302","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Expression of R-spondins/Lgrs in development of movable craniofacial organs
Wnt signaling plays a critical role in the development of many organs, including the major movable craniofacial organs tongue, lip, and eyelid. Four members of the R-spondin family (Rspo1–4) bind to Lgr4/5/6 to regulate the activation of Wnt signaling. However, it is not fully understood how Rspos/Lgrs regulate Wnt signaling during the development of movable craniofacial organs. To address this question, we examined the expression of Rspos, Lgrs, and Axin2 (major mediator of canonical Wnt signaling) during tongue, lip, and eyelid development. The expression of Axin2, Rspos and Lgrs was observed in many similar regions, suggesting that Rspos likely activate canonical Wnt signaling through the Lgr-dependent pathway in these regions. Lgr expression was not detected in regions where Axin2 and Rspos were expressed, suggesting that Rspos might activate canonical Wnt signaling through the Lgr-independent pathway in these regions. In addition, the expression of Rspos and Lgrs were observed in some other regions where Axin2 was not expressed, suggesting the possibility that Rspos and/or Lgrs are involved in non-canonical Wnt signaling or the Wnt-independent pathway. Thus, we identified a dynamic spatiotemporal expression pattern of Rspos and Lgrs during the development of the eyelid, tongue, and lip.
期刊介绍:
Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include:
-In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression
-Temporal studies of large gene sets during development
-Transgenic studies to study cell lineage in tissue formation