补充鱼油对九只健康狗外周血淋巴细胞 DNA 损伤的影响。

IF 1.3 Q2 VETERINARY SCIENCES
Veterinary Record Open Pub Date : 2021-06-22 eCollection Date: 2021-12-01 DOI:10.1002/vro2.12
Francisco J Pellegrino, Analía Risso, Yanina Corrada, Rocío C Gambaro, Analía I Seoane
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引用次数: 0

摘要

背景:补充鱼油(FO)作为欧米伽 3 脂肪酸的来源,对健康有益。然而,膳食中不饱和脂肪酸含量过高会导致脂质过氧化和蛋白质、脂质和 DNA 损伤增加。我们评估了膳食中补充 FO 对狗外周血淋巴细胞 DNA 损伤的影响。此外,我们还确定了补充 FO 对这些狗的脂质过氧化反应和脂质概况的影响。方法:将健康雄性狗(n = 9)随机分配到两种饮食中的一种,为期 90 天:对照组(CG,n = 4),以商业食品为基础;FO(FOG,n = 5),在相同食品中补充 1000 毫克 FO。在第 1 天、第 30 天、第 60 天和第 90 天采集血液样本。用彗星试验评估 DNA 损伤,并得出损伤指数。丙二醛(MDA)水平的测定是脂质过氧化的指标。血脂谱测定包括血清甘油三酯、胆固醇、低密度脂蛋白和高密度脂蛋白(HDL)水平:与 CG 相比,FOG 在第 30 天(CG,13.7 ± 2.5;FOG,6.5 ± 2.5)、第 60 天(CG,14.7 ± 2.5;FOG,3.5 ± 2.5)和第 90 天(CG,15.5 ± 2.5;FOG,3.0 ± 2.5)的损伤指数值(任意单位)较低(处理 × 时间交互作用,p p 结论):这些研究结果表明,膳食中补充 FO 不会诱发健康狗外周血淋巴细胞的 DNA 损伤,反而会减少这种损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs.

Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs.

Background: Fish oil (FO) supplementation as a source of omega 3 fatty acids is associated with beneficial effects on health. However, high unsaturated fatty acid content in the diet could result in increased lipid peroxidation and damage to proteins, lipids and DNA. We evaluated the effect of dietary FO supplementation on DNA damage in peripheral blood lymphocytes of dogs. Additionally, we determined the effect of FO supplementation on lipid peroxidation and lipid profile of these dogs.

Methods: Healthy male dogs (n = 9) were randomly assigned to one of two diets during 90 days: control (CG, n = 4), based on a commercial food, and FO (FOG, n = 5), the same food supplemented with 1000 mg FO. Blood samples were collected on days -1, 30, 60 and 90. DNA damage was assessed with the comet assay, and the damage index was obtained. Malondialdehyde (MDA) levels were determined as an indicator of lipid peroxidation. Lipid profile determination included serum triglyceride, cholesterol, low-density lipoprotein and high-density lipoprotein levels (HDL).

Results: Damage index values (arbitrary units) were lower in FOG on day 30 (CG, 13.7 ± 2.5; FOG, 6.5 ± 2.5), 60 (CG, 14.7 ± 2.5; FOG, 3.5 ± 2.5) and 90 (CG, 15.5 ± 2.5; FOG, 3.0 ± 2.5) compared with CG (treatment × time interaction, p < 0.01). Serum MDA and HDL concentrations were lower in FOG compared with CG on day 60 and 90 (treatment × time interaction, p < 0.05).

Conclusion: These findings suggest that dietary FO supplementation did not induce DNA damage in peripheral blood lymphocytes of healthy dogs, but rather reduced it.

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来源期刊
Veterinary Record Open
Veterinary Record Open VETERINARY SCIENCES-
CiteScore
3.00
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: Veterinary Record Open is a journal dedicated to publishing specialist veterinary research across a range of topic areas including those of a more niche and specialist nature to that considered in the weekly Vet Record. Research from all disciplines of veterinary interest will be considered. It is an Open Access journal of the British Veterinary Association.
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