修复术一种修复和保存已安装的解剖病理标本的方法

IF 0.6 4区 生物学 Q4 CELL BIOLOGY
Journal of Histotechnology Pub Date : 2021-09-01 Epub Date: 2021-05-17 DOI:10.1080/01478885.2021.1884352
Manisha Kulkarni, Pradeep Vaideeswar
{"title":"修复术一种修复和保存已安装的解剖病理标本的方法","authors":"Manisha Kulkarni,&nbsp;Pradeep Vaideeswar","doi":"10.1080/01478885.2021.1884352","DOIUrl":null,"url":null,"abstract":"<p><p>Big hospitals and biology museums often have a sizable collection of valuable archived anatomical pathology specimens, collected over a long period of time. Traditionally, these specimens are suspended by thread tied to a frame of glass rods in a rectangular glass jar. While restoring with preserving solution, specimens often fell from sewn thread or strings. We developed an easy method of remounting such specimens on Perspex sheets with cyanoacrylate adhesive. This technique was tested on five archived specimens and five recent well-fixed specimens, including brain. Specimens could be easily oriented, remounted and remained well adhered to the Perspex sheet. Gross pathologic characteristics could be well illustrated from all sides in the remounted specimen. Cyanoacrylate adhesive technique was useful in permanent preservation of old delicate anatomical pathology specimens, which could not be tied to glass rods again and had fallen out of position in mounting jars. This technique can also be applied for well-fixed recent specimens. It is easy, cost effective, serves as an educational tool and can be applied to all types of specimens.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"144-149"},"PeriodicalIF":0.6000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2021.1884352","citationCount":"0","resultStr":"{\"title\":\"A method of restoration and preservation of mounted anatomical pathology specimens.\",\"authors\":\"Manisha Kulkarni,&nbsp;Pradeep Vaideeswar\",\"doi\":\"10.1080/01478885.2021.1884352\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Big hospitals and biology museums often have a sizable collection of valuable archived anatomical pathology specimens, collected over a long period of time. Traditionally, these specimens are suspended by thread tied to a frame of glass rods in a rectangular glass jar. While restoring with preserving solution, specimens often fell from sewn thread or strings. We developed an easy method of remounting such specimens on Perspex sheets with cyanoacrylate adhesive. This technique was tested on five archived specimens and five recent well-fixed specimens, including brain. Specimens could be easily oriented, remounted and remained well adhered to the Perspex sheet. Gross pathologic characteristics could be well illustrated from all sides in the remounted specimen. Cyanoacrylate adhesive technique was useful in permanent preservation of old delicate anatomical pathology specimens, which could not be tied to glass rods again and had fallen out of position in mounting jars. This technique can also be applied for well-fixed recent specimens. It is easy, cost effective, serves as an educational tool and can be applied to all types of specimens.</p>\",\"PeriodicalId\":15966,\"journal\":{\"name\":\"Journal of Histotechnology\",\"volume\":\"44 3\",\"pages\":\"144-149\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2021-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/01478885.2021.1884352\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Histotechnology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/01478885.2021.1884352\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/5/17 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Histotechnology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/01478885.2021.1884352","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/5/17 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

大型医院和生物博物馆通常有相当数量的有价值的解剖病理标本存档,这些标本是在很长一段时间内收集的。传统上,这些标本是用线悬挂在一个矩形的玻璃罐中,绳子系在玻璃棒的框架上。在用保存液修复时,标本经常从缝纫的线或绳子上掉下来。我们开发了一种简单的方法,用氰基丙烯酸酯胶粘剂将这些标本重新安装在有机玻璃片上。这项技术在5个存档标本和5个最近修复好的标本上进行了测试,其中包括大脑。样品可以很容易地定向,重新安装,并保持良好的粘接在有机玻璃板上。在重新安装的标本中,大体病理特征可以从各个方面得到很好的说明。氰基丙烯酸酯粘接技术适用于永久保存旧的精细解剖病理标本,这些标本不能再次绑在玻璃棒上,并且在安装罐中脱落。该技术也可用于固定良好的新标本。它简单,成本效益高,可作为一种教育工具,可应用于所有类型的标本。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A method of restoration and preservation of mounted anatomical pathology specimens.

Big hospitals and biology museums often have a sizable collection of valuable archived anatomical pathology specimens, collected over a long period of time. Traditionally, these specimens are suspended by thread tied to a frame of glass rods in a rectangular glass jar. While restoring with preserving solution, specimens often fell from sewn thread or strings. We developed an easy method of remounting such specimens on Perspex sheets with cyanoacrylate adhesive. This technique was tested on five archived specimens and five recent well-fixed specimens, including brain. Specimens could be easily oriented, remounted and remained well adhered to the Perspex sheet. Gross pathologic characteristics could be well illustrated from all sides in the remounted specimen. Cyanoacrylate adhesive technique was useful in permanent preservation of old delicate anatomical pathology specimens, which could not be tied to glass rods again and had fallen out of position in mounting jars. This technique can also be applied for well-fixed recent specimens. It is easy, cost effective, serves as an educational tool and can be applied to all types of specimens.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Histotechnology
Journal of Histotechnology 生物-细胞生物学
CiteScore
2.60
自引率
9.10%
发文量
30
审稿时长
>12 weeks
期刊介绍: The official journal of the National Society for Histotechnology, Journal of Histotechnology, aims to advance the understanding of complex biological systems and improve patient care by applying histotechniques to diagnose, prevent and treat diseases. Journal of Histotechnology is concerned with educating practitioners and researchers from diverse disciplines about the methods used to prepare tissues and cell types, from all species, for microscopic examination. This is especially relevant to Histotechnicians. Journal of Histotechnology welcomes research addressing new, improved, or traditional techniques for tissue and cell preparation. This includes review articles, original articles, technical notes, case studies, advances in technology, and letters to editors. Topics may include, but are not limited to, discussion of clinical, veterinary, and research histopathology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信