用于伤口愈合的多草药组合:洋甘菊和石榴

Somayeh Niknam, Zahra Tofighi, Mohammad Ali Faramarzi, Mohammad Amin Abdollahifar, Ensieh Sajadi, Rassoul Dinarvand, Tayebeh Toliyat
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引用次数: 0

摘要

背景:具有收敛活性的石榴(Punica granatum L.)和具有抗炎和抗氧化特性的洋甘菊(Matricaria chamomilla L.)是治疗各种皮肤疾病(包括伤口愈合)的天然药物:本研究旨在评估石榴花和洋甘菊花甲醇提取物的单独和联合伤口愈合活性:方法:制备石榴花和洋甘菊花的薄荷醇馏分后,测定馏分中总酚、总鞣质和总黄酮类化合物的含量。为了对石榴和洋甘菊馏分进行标准化,使用高效液相色谱法(HPLC)测定了它们的没食子酸和芹菜素-7-O-葡萄糖苷含量。此外,还使用 DPPH 和 FRAP 试验检测了它们的抗氧化活性。对金黄色葡萄球菌、表皮葡萄球菌和绿脓杆菌进行了抗菌试验。在大鼠背部切除伤口模型中,研究了每种植物的三种不同浓度的甲醇馏分和一种馏分组合剂量的伤口愈合活性。最后,在实验结束时进行了组织病理学研究:植物化学检查显示,石榴花中含有大量酚类化合物,而甘菊花馏分则含有大量总黄酮。两种馏分,尤其是石榴花,都具有很强的抗氧化活性。伤口诱导 7 天后,伤口闭合效果最佳。在所有测量时间内,所有治疗组的伤口收缩效果都优于安慰剂组。与单一疗法相比,石榴和甘菊的组合疗法具有更好的伤口愈合特性,尤其是在伤口诱导的早期:本研究表明,石榴花和洋甘菊花的甲醇馏分具有较高的抗氧化和伤口愈合活性,这可能与它们的植物化学成分含量较高有关。与单一草药治疗相比,这两种馏分的低浓度组合形式可加速伤口愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Polyherbal combination for wound healing: Matricaria chamomilla L. and Punica granatum L.

Background: Punica granatum L. (pomegranate) with astringent activities and Matricaria chamomilla L. (chamomile) with anti-inflammatory and antioxidant properties are natural remedies used for various skin disorders, including wound healing.

Objectives: This study was conducted to evaluate the individual and combined wound healing activity of the methanol extracts of pomegranate and chamomile flowers.

Methods: After preparing the menthol fraction of pomegranate and chamomile flowers, the content of total phenols, total tannins, and total flavonoids of fractions was measured. For standardization of pomegranate and chamomile fractions, Gallic acid and apigenin-7-O-glucoside contents of them were determined using high-performance liquid chromatography (HPLC). Moreover, their antioxidant activities were examined using DPPH and FRAP tests. The antimicrobial assay was performed against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Three different concentrations of methanol fraction of each plant and one combination dose of fractions were investigated for their wound healing activities in an excision wound model on the rats' dorsum. Finally, histopathological studies were done at the end of the experiment.

Results: Phytochemical examinations showed high amounts of phenolic compounds in pomegranate flowers, while chamomile flower fractions contained a high amount of total flavonoids. Both fractions, especially pomegranate, had potent antioxidant activity. The best results for wound closure were observed 7 days after wound induction. All treated groups exhibited superior wound contraction compared to their placebo at all measurement times. The combined form of pomegranate and chamomile had better wound healing properties compared to a single therapy, especially on time earlier to wound induction.

Conclusion: This study represented high antioxidant and wound healing activities for methanol fraction of pomegranate and chamomile flowers, which could be related to their high content of phytochemicals. In comparison with single herb treatment, the combined form of these two fractions in lower concentrations accelerated wound closure.

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