Carolina L. Alcantara, Wanderley de Souza, Narcisa L. Cunha e Silva
{"title":"克氏锥虫胞内和胞外无尾线虫的胞口-细胞咽复合体在结构和功能上存在差异","authors":"Carolina L. Alcantara, Wanderley de Souza, Narcisa L. Cunha e Silva","doi":"10.1111/cmi.13346","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <p>Endocytosis in <i>Trypanosoma cruzi</i> is mainly performed through a specialised membrane domain called cytostome-cytopharynx complex. Its ultrastructure and dynamics in endocytosis are well characterized in epimastigotes, being absent in trypomastigotes, that lack endocytic activity. Intracellular amastigotes also possess a cytostome-cytopharynx but participation in endocytosis of these forms is not clear. Extracellular amastigotes can be obtained from the supernatant of infected cells or in vitro amastigogenesis. These amastigotes share biochemical and morphological features with intracellular amastigotes but retain trypomastigote's ability to establish infection. We analysed and compared the ultrastructure of the cytostome-cytopharynx complex of intracellular amastigotes and extracellular amastigotes using high-resolution tridimensional electron microscopy techniques. We compared the endocytic ability of intracellular amastigotes, obtained through host cell lysis, with that of extracellular amastigotes. Intracellular amastigotes showed a cytostome-cytopharynx complex similar to epimastigotes'. However, after isolation, the complex undergoes ultrastructural modifications that progressively took to an impairment of endocytosis. Extracellular amastigotes do not possess a cytostome-cytopharynx complex nor the ability to endocytose. Those observations highlight morpho functional differences between intra and extracellular amastigotes regarding an important structure related to cell metabolism.</p>\n </section>\n \n <section>\n \n <h3> Take Aways</h3>\n \n <div>\n <ul>\n \n <li><i>T. cruzi</i> intracellular amastigotes endocytose through the cytostome-cytopharynx complex.</li>\n \n <li>The cytostome-cytopharynx complex of intracellular amastigotes is ultrastructurally similar to the epimastigote.</li>\n \n <li>Intracellular amastigotes, once outside the host cell, disassembles the cytostome-cytopharynx membrane domain.</li>\n \n <li>Extracellular amastigotes do not possess a cytostome-cytopharynx either the ability to endocytose.</li>\n </ul>\n </div>\n </section>\n </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"23 9","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2021-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/cmi.13346","citationCount":"5","resultStr":"{\"title\":\"The cytostome-cytopharynx complex of intracellular and extracellular amastigotes of Trypanosoma cruzi exhibit structural and functional differences\",\"authors\":\"Carolina L. Alcantara, Wanderley de Souza, Narcisa L. Cunha e Silva\",\"doi\":\"10.1111/cmi.13346\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <p>Endocytosis in <i>Trypanosoma cruzi</i> is mainly performed through a specialised membrane domain called cytostome-cytopharynx complex. Its ultrastructure and dynamics in endocytosis are well characterized in epimastigotes, being absent in trypomastigotes, that lack endocytic activity. Intracellular amastigotes also possess a cytostome-cytopharynx but participation in endocytosis of these forms is not clear. Extracellular amastigotes can be obtained from the supernatant of infected cells or in vitro amastigogenesis. These amastigotes share biochemical and morphological features with intracellular amastigotes but retain trypomastigote's ability to establish infection. We analysed and compared the ultrastructure of the cytostome-cytopharynx complex of intracellular amastigotes and extracellular amastigotes using high-resolution tridimensional electron microscopy techniques. We compared the endocytic ability of intracellular amastigotes, obtained through host cell lysis, with that of extracellular amastigotes. Intracellular amastigotes showed a cytostome-cytopharynx complex similar to epimastigotes'. However, after isolation, the complex undergoes ultrastructural modifications that progressively took to an impairment of endocytosis. Extracellular amastigotes do not possess a cytostome-cytopharynx complex nor the ability to endocytose. Those observations highlight morpho functional differences between intra and extracellular amastigotes regarding an important structure related to cell metabolism.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Take Aways</h3>\\n \\n <div>\\n <ul>\\n \\n <li><i>T. cruzi</i> intracellular amastigotes endocytose through the cytostome-cytopharynx complex.</li>\\n \\n <li>The cytostome-cytopharynx complex of intracellular amastigotes is ultrastructurally similar to the epimastigote.</li>\\n \\n <li>Intracellular amastigotes, once outside the host cell, disassembles the cytostome-cytopharynx membrane domain.</li>\\n \\n <li>Extracellular amastigotes do not possess a cytostome-cytopharynx either the ability to endocytose.</li>\\n </ul>\\n </div>\\n </section>\\n </div>\",\"PeriodicalId\":9844,\"journal\":{\"name\":\"Cellular Microbiology\",\"volume\":\"23 9\",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2021-04-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/cmi.13346\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cellular Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/cmi.13346\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cellular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/cmi.13346","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
The cytostome-cytopharynx complex of intracellular and extracellular amastigotes of Trypanosoma cruzi exhibit structural and functional differences
Endocytosis in Trypanosoma cruzi is mainly performed through a specialised membrane domain called cytostome-cytopharynx complex. Its ultrastructure and dynamics in endocytosis are well characterized in epimastigotes, being absent in trypomastigotes, that lack endocytic activity. Intracellular amastigotes also possess a cytostome-cytopharynx but participation in endocytosis of these forms is not clear. Extracellular amastigotes can be obtained from the supernatant of infected cells or in vitro amastigogenesis. These amastigotes share biochemical and morphological features with intracellular amastigotes but retain trypomastigote's ability to establish infection. We analysed and compared the ultrastructure of the cytostome-cytopharynx complex of intracellular amastigotes and extracellular amastigotes using high-resolution tridimensional electron microscopy techniques. We compared the endocytic ability of intracellular amastigotes, obtained through host cell lysis, with that of extracellular amastigotes. Intracellular amastigotes showed a cytostome-cytopharynx complex similar to epimastigotes'. However, after isolation, the complex undergoes ultrastructural modifications that progressively took to an impairment of endocytosis. Extracellular amastigotes do not possess a cytostome-cytopharynx complex nor the ability to endocytose. Those observations highlight morpho functional differences between intra and extracellular amastigotes regarding an important structure related to cell metabolism.
Take Aways
T. cruzi intracellular amastigotes endocytose through the cytostome-cytopharynx complex.
The cytostome-cytopharynx complex of intracellular amastigotes is ultrastructurally similar to the epimastigote.
Intracellular amastigotes, once outside the host cell, disassembles the cytostome-cytopharynx membrane domain.
Extracellular amastigotes do not possess a cytostome-cytopharynx either the ability to endocytose.
期刊介绍：
Cellular Microbiology aims to publish outstanding contributions to the understanding of interactions between microbes, prokaryotes and eukaryotes, and their host in the context of pathogenic or mutualistic relationships, including co-infections and microbiota. We welcome studies on single cells, animals and plants, and encourage the use of model hosts and organoid cultures. Submission on cell and molecular biological aspects of microbes, such as their intracellular organization or the establishment and maintenance of their architecture in relation to virulence and pathogenicity are also encouraged. Contributions must provide mechanistic insights supported by quantitative data obtained through imaging, cellular, biochemical, structural or genetic approaches.
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