出生时DNA甲基化与儿童血清免疫球蛋白E水平相关

IF 3.2 Q2 GENETICS & HEREDITY
Epigenetics Insights Pub Date : 2021-04-05 eCollection Date: 2021-01-01 DOI:10.1177/25168657211008108
Luhang Han, Akhilesh Kaushal, Hongmei Zhang, Latha Kadalayil, Jiasong Duan, John W Holloway, Wilfried Karmaus, Pratik Banerjee, Shih-Fen Tsai, Hui-Ju Wen, Syed Hasan Arshad, Shu-Li Wang
{"title":"出生时DNA甲基化与儿童血清免疫球蛋白E水平相关","authors":"Luhang Han,&nbsp;Akhilesh Kaushal,&nbsp;Hongmei Zhang,&nbsp;Latha Kadalayil,&nbsp;Jiasong Duan,&nbsp;John W Holloway,&nbsp;Wilfried Karmaus,&nbsp;Pratik Banerjee,&nbsp;Shih-Fen Tsai,&nbsp;Hui-Ju Wen,&nbsp;Syed Hasan Arshad,&nbsp;Shu-Li Wang","doi":"10.1177/25168657211008108","DOIUrl":null,"url":null,"abstract":"<p><p>Immunoglobulin E (IgE) is known to play an important role in allergic diseases. Epigenetic traits acquired due to modification of deoxyribonucleic acid (DNA) methylation (DNAm) in early life may have phenotypic consequences through their role in transcriptional regulation with relevance to the developmental origins of diseases including allergy. However, epigenome-scale studies on the longitudinal association of cord blood DNAm with IgE over time are lacking. Our study aimed to examine the association of DNAm at birth with childhood serum IgE levels during early life. Genome-scale DNAm and total serum IgE measured at birth, 5, 8, and 11 years of children in the Taiwan Maternal and Infant Cohort Study were included in the study in the discovery stage. Linear mixed models were implemented to assess the association between cord blood DNAm at ~310K 5'-cytosine-phosphate-guanine-3' (CpG) sites with repeated IgE measurements, adjusting for cord blood IgE. Identified statistically significant CpGs (at a false discovery rate, FDR, of 0.05) were further tested in an independent replication cohort, the Isle of Wight (IoW) birth cohort. We mapped replicated CpGs to genes and conducted gene ontology analysis using ToppFun to identify significantly enriched pathways and biological processes of the genes. Cord blood DNAm of 273 CpG sites were significantly (FDR = 0.05) associated with IgE levels longitudinally. Among the identified CpGs available in both cohorts (184 CpGs), 92 CpGs (50%) were replicated in the IoW in terms of consistency in direction of associations between DNA methylation and IgE levels later in life, and 16 of the 92 CpGs showed statistically significant associations (<i>P</i> < .05). Gene ontology analysis identified 4 pathways (FDR = 0.05). The identified 16 CpG sites had the potential to serve as epigenetic markers associated with later IgE production, beneficial to allergic disease prevention and intervention.</p>","PeriodicalId":41996,"journal":{"name":"Epigenetics Insights","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2021-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/25168657211008108","citationCount":"7","resultStr":"{\"title\":\"DNA Methylation at Birth is Associated with Childhood Serum Immunoglobulin E Levels.\",\"authors\":\"Luhang Han,&nbsp;Akhilesh Kaushal,&nbsp;Hongmei Zhang,&nbsp;Latha Kadalayil,&nbsp;Jiasong Duan,&nbsp;John W Holloway,&nbsp;Wilfried Karmaus,&nbsp;Pratik Banerjee,&nbsp;Shih-Fen Tsai,&nbsp;Hui-Ju Wen,&nbsp;Syed Hasan Arshad,&nbsp;Shu-Li Wang\",\"doi\":\"10.1177/25168657211008108\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Immunoglobulin E (IgE) is known to play an important role in allergic diseases. Epigenetic traits acquired due to modification of deoxyribonucleic acid (DNA) methylation (DNAm) in early life may have phenotypic consequences through their role in transcriptional regulation with relevance to the developmental origins of diseases including allergy. However, epigenome-scale studies on the longitudinal association of cord blood DNAm with IgE over time are lacking. Our study aimed to examine the association of DNAm at birth with childhood serum IgE levels during early life. Genome-scale DNAm and total serum IgE measured at birth, 5, 8, and 11 years of children in the Taiwan Maternal and Infant Cohort Study were included in the study in the discovery stage. Linear mixed models were implemented to assess the association between cord blood DNAm at ~310K 5'-cytosine-phosphate-guanine-3' (CpG) sites with repeated IgE measurements, adjusting for cord blood IgE. Identified statistically significant CpGs (at a false discovery rate, FDR, of 0.05) were further tested in an independent replication cohort, the Isle of Wight (IoW) birth cohort. We mapped replicated CpGs to genes and conducted gene ontology analysis using ToppFun to identify significantly enriched pathways and biological processes of the genes. Cord blood DNAm of 273 CpG sites were significantly (FDR = 0.05) associated with IgE levels longitudinally. Among the identified CpGs available in both cohorts (184 CpGs), 92 CpGs (50%) were replicated in the IoW in terms of consistency in direction of associations between DNA methylation and IgE levels later in life, and 16 of the 92 CpGs showed statistically significant associations (<i>P</i> < .05). Gene ontology analysis identified 4 pathways (FDR = 0.05). The identified 16 CpG sites had the potential to serve as epigenetic markers associated with later IgE production, beneficial to allergic disease prevention and intervention.</p>\",\"PeriodicalId\":41996,\"journal\":{\"name\":\"Epigenetics Insights\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2021-04-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1177/25168657211008108\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Epigenetics Insights\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1177/25168657211008108\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Epigenetics Insights","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/25168657211008108","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 7

摘要

免疫球蛋白E (IgE)在过敏性疾病中起着重要作用。由于生命早期脱氧核糖核酸(DNA)甲基化(DNAm)修饰而获得的表观遗传性状可能通过其在转录调控中的作用具有表型后果,与过敏等疾病的发育起源相关。然而,关于脐带血dna与IgE随时间的纵向关联的表观基因组尺度研究尚缺乏。我们的研究旨在研究出生时dna与儿童早期血清IgE水平的关系。本研究在发现阶段将台湾母婴队列研究中出生、5岁、8岁和11岁儿童的基因组尺度DNAm和血清总IgE纳入研究。采用线性混合模型评估脐带血dna在~310K 5′-胞嘧啶-磷酸-鸟嘌呤-3′(CpG)位点与重复IgE测量之间的关系,调整脐带血IgE。在一个独立的复制队列,怀特岛(IoW)出生队列中进一步测试了具有统计学意义的CpGs(错误发现率,FDR, 0.05)。我们将复制的CpGs定位到基因上,并使用ToppFun进行基因本体分析,以确定基因的显著富集途径和生物学过程。脐带血273个CpG位点的dna与IgE水平呈显著的纵向相关性(FDR = 0.05)。在两个队列中可用的鉴定CpGs(184个CpGs)中,就DNA甲基化与生命后期IgE水平之间的关联方向的一致性而言,92个CpGs(50%)在IoW中被复制,并且92个CpGs中有16个显示出统计学上显著的相关性(P
本文章由计算机程序翻译,如有差异,请以英文原文为准。

DNA Methylation at Birth is Associated with Childhood Serum Immunoglobulin E Levels.

DNA Methylation at Birth is Associated with Childhood Serum Immunoglobulin E Levels.

DNA Methylation at Birth is Associated with Childhood Serum Immunoglobulin E Levels.

DNA Methylation at Birth is Associated with Childhood Serum Immunoglobulin E Levels.

Immunoglobulin E (IgE) is known to play an important role in allergic diseases. Epigenetic traits acquired due to modification of deoxyribonucleic acid (DNA) methylation (DNAm) in early life may have phenotypic consequences through their role in transcriptional regulation with relevance to the developmental origins of diseases including allergy. However, epigenome-scale studies on the longitudinal association of cord blood DNAm with IgE over time are lacking. Our study aimed to examine the association of DNAm at birth with childhood serum IgE levels during early life. Genome-scale DNAm and total serum IgE measured at birth, 5, 8, and 11 years of children in the Taiwan Maternal and Infant Cohort Study were included in the study in the discovery stage. Linear mixed models were implemented to assess the association between cord blood DNAm at ~310K 5'-cytosine-phosphate-guanine-3' (CpG) sites with repeated IgE measurements, adjusting for cord blood IgE. Identified statistically significant CpGs (at a false discovery rate, FDR, of 0.05) were further tested in an independent replication cohort, the Isle of Wight (IoW) birth cohort. We mapped replicated CpGs to genes and conducted gene ontology analysis using ToppFun to identify significantly enriched pathways and biological processes of the genes. Cord blood DNAm of 273 CpG sites were significantly (FDR = 0.05) associated with IgE levels longitudinally. Among the identified CpGs available in both cohorts (184 CpGs), 92 CpGs (50%) were replicated in the IoW in terms of consistency in direction of associations between DNA methylation and IgE levels later in life, and 16 of the 92 CpGs showed statistically significant associations (P < .05). Gene ontology analysis identified 4 pathways (FDR = 0.05). The identified 16 CpG sites had the potential to serve as epigenetic markers associated with later IgE production, beneficial to allergic disease prevention and intervention.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Epigenetics Insights
Epigenetics Insights GENETICS & HEREDITY-
CiteScore
5.10
自引率
0.00%
发文量
10
审稿时长
8 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信