白细胞介素-6促进牛囊胚原始内胚层发育。

Q2 Biochemistry, Genetics and Molecular Biology
Lydia K Wooldridge, Alan D Ealy
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引用次数: 1

摘要

背景:白细胞介素-6 (IL6)最近被确定为牛胚胎中的一种胚胎营养因子,其主要作用是调节内细胞质量(ICM)的大小。本研究探讨了IL6是否影响外胚层(EPI)和原始内胚层(PE)的发育,这是ICM形成后产生的两种胚胎谱系。EPI核标记物(NANOG)和PE核标记物(GATA6)用于区分两种细胞类型。结论:总之,在EPI/PE细胞命运建立后,IL6操纵ICM的发展。PE细胞是IL6的靶标,其中依赖于jak的信号用于调节PE数量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Interleukin-6 promotes primitive endoderm development in bovine blastocysts.

Interleukin-6 promotes primitive endoderm development in bovine blastocysts.

Interleukin-6 promotes primitive endoderm development in bovine blastocysts.

Interleukin-6 promotes primitive endoderm development in bovine blastocysts.

Background: Interleukin-6 (IL6) was recently identified as an embryotrophic factor in bovine embryos, where it acts primarily to mediate inner cell mass (ICM) size. This work explored whether IL6 affects epiblast (EPI) and primitive endoderm (PE) development, the two embryonic lineages generated from the ICM after its formation. Nuclear markers for EPI (NANOG) and PE (GATA6) were used to differentiate the two cell types.

Results: Increases (P < 0.05) in total ICM cell numbers and PE cell numbers were detected in bovine blastocysts at day 8 and 9 post-fertilization after exposure to 100 ng/ml recombinant bovine IL6. Also, IL6 increased (P < 0.05) the number of undifferentiated ICM cells (cells containing both PE and EPI markers). The effects of IL6 on EPI cell numbers were inconsistent. Studies were also completed to explore the importance of Janus kinase 2 (JAK2)-dependent signaling in bovine PE cells. Definitive activation of STAT3, a downstream target for JAK2, was observed in PE cells. Also, pharmacological inhibition of JAK2 decreased (P < 0.05) PE cell numbers.

Conclusions: To conclude, IL6 manipulates ICM development after EPI/PE cell fates are established. The PE cells are the target for IL6, where a JAK-dependent signal is used to regulate PE numbers.

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来源期刊
BMC Developmental Biology
BMC Developmental Biology 生物-发育生物学
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: BMC Developmental Biology is an open access, peer-reviewed journal that considers articles on the development, growth, differentiation and regeneration of multicellular organisms, including molecular, cellular, tissue, organ and whole organism research.
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