牛肝菌(Newbouldia laevis)和决明子(Cassia tora)叶片内生真菌的次级代谢产物:寻找新的抗菌剂。

Q3 Medicine
Anthonia Adaeze Amaechi, Angus Nnamdi Oli, Ugochukwu Moses Okezie, Samson A. Adejumo, Chika Christiana Abba, Ifeanyi Justin Okeke, Festus Basden Chiedu Okoye
{"title":"牛肝菌(Newbouldia laevis)和决明子(Cassia tora)叶片内生真菌的次级代谢产物:寻找新的抗菌剂。","authors":"Anthonia Adaeze Amaechi, Angus Nnamdi Oli, Ugochukwu Moses Okezie, Samson A. Adejumo, Chika Christiana Abba, Ifeanyi Justin Okeke, Festus Basden Chiedu Okoye","doi":"10.2174/1574891X15999201222152646","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>This study tries to prospect for new antimicrobial agents using some Nigerian plants Background: Antimicrobial compounds from fungi endophytes have shown great promise in mitigating the threats of resistant pathogens.</p><p><strong>Objective: </strong>The study evaluated the in vitro antimicrobial property of secondary metabolites of endophytic fungi isolated from Newbouldia laevis and Cassia tora leaves.</p><p><strong>Methods: </strong>Ten endophytic fungi were isolated from the two plants' leaves and later fermented on local rice for 21 days. Thereafter, their secondary metabolites were extracted using ethyl acetate. The antibacterial activity of the extracts on the test organisms were determined using agar diffusion and agar dilution methods, while the bioactive constituents were identified using High performance liquid chromatography coupled to diode array detector.</p><p><strong>Results: </strong>Nine of the crude extracts (NL1, NL3, NL6, NL10, NL12, CT2, CT7, CT9 and CT10) of the fungi isolates inhibited at least one of the microorganisms studied with maximum and minimum Inhibition-Zone-Diameter of 14 mm and 2 mm respectively while CT1 did not inhibit any of the tested microorganisms at tested concentrations. The extracts exhibited good antifungal activity, inhibiting the growth of both C. albicans and Trichophyton tested with an InhibitionZone-Diameter ranging between 4-8 mm and 7-14 mm respectively. The endophytic fungi extracts- CT2 and NL1- exhibited the best antimicrobial activity, inhibiting most of the tested microorganisms. HPLC-DAD analysis of the endophytic fungal extracts identified some classes of compounds such as catechin derivatives, benzoic acid derivatives and apigenin, which were previously reported to have antimicrobial potentials.</p><p><strong>Conclusion: </strong>Newbouldia laevis and Cassia tora leaves house endophytic fungi capable of yielding secondary metabolites with potential as anti-infective agents.</p>","PeriodicalId":20909,"journal":{"name":"Recent patents on anti-infective drug discovery","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Secondary metabolites of endophytic fungi from Newbouldia laevis and Cassia tora leaves: prospecting for new antimicrobial agents.\",\"authors\":\"Anthonia Adaeze Amaechi, Angus Nnamdi Oli, Ugochukwu Moses Okezie, Samson A. Adejumo, Chika Christiana Abba, Ifeanyi Justin Okeke, Festus Basden Chiedu Okoye\",\"doi\":\"10.2174/1574891X15999201222152646\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>This study tries to prospect for new antimicrobial agents using some Nigerian plants Background: Antimicrobial compounds from fungi endophytes have shown great promise in mitigating the threats of resistant pathogens.</p><p><strong>Objective: </strong>The study evaluated the in vitro antimicrobial property of secondary metabolites of endophytic fungi isolated from Newbouldia laevis and Cassia tora leaves.</p><p><strong>Methods: </strong>Ten endophytic fungi were isolated from the two plants' leaves and later fermented on local rice for 21 days. Thereafter, their secondary metabolites were extracted using ethyl acetate. The antibacterial activity of the extracts on the test organisms were determined using agar diffusion and agar dilution methods, while the bioactive constituents were identified using High performance liquid chromatography coupled to diode array detector.</p><p><strong>Results: </strong>Nine of the crude extracts (NL1, NL3, NL6, NL10, NL12, CT2, CT7, CT9 and CT10) of the fungi isolates inhibited at least one of the microorganisms studied with maximum and minimum Inhibition-Zone-Diameter of 14 mm and 2 mm respectively while CT1 did not inhibit any of the tested microorganisms at tested concentrations. The extracts exhibited good antifungal activity, inhibiting the growth of both C. albicans and Trichophyton tested with an InhibitionZone-Diameter ranging between 4-8 mm and 7-14 mm respectively. The endophytic fungi extracts- CT2 and NL1- exhibited the best antimicrobial activity, inhibiting most of the tested microorganisms. HPLC-DAD analysis of the endophytic fungal extracts identified some classes of compounds such as catechin derivatives, benzoic acid derivatives and apigenin, which were previously reported to have antimicrobial potentials.</p><p><strong>Conclusion: </strong>Newbouldia laevis and Cassia tora leaves house endophytic fungi capable of yielding secondary metabolites with potential as anti-infective agents.</p>\",\"PeriodicalId\":20909,\"journal\":{\"name\":\"Recent patents on anti-infective drug discovery\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-12-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Recent patents on anti-infective drug discovery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1574891X15999201222152646\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Recent patents on anti-infective drug discovery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1574891X15999201222152646","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

摘要

目的:本研究试图利用尼日利亚的一些植物寻找新的抗菌剂:来自真菌内生菌的抗菌化合物在减轻抗药性病原体的威胁方面大有可为:本研究评估了从 Newbouldia laevis 和 Cassia tora 叶子中分离出来的内生真菌次生代谢产物的体外抗菌特性:从这两种植物的叶片中分离出 10 种内生真菌,然后用当地大米发酵 21 天。然后,用乙酸乙酯提取它们的次级代谢产物。采用琼脂扩散法和琼脂稀释法测定提取物对受试生物的抗菌活性,并采用高效液相色谱法和二极管阵列检测器鉴定生物活性成分:九种真菌分离物的粗提取物(NL1、NL3、NL6、NL10、NL12、CT2、CT7、CT9 和 CT10)至少抑制了一种微生物,最大和最小抑制区直径分别为 14 毫米和 2 毫米,而 CT1 在测试浓度下未抑制任何受测微生物。提取物表现出良好的抗真菌活性,可抑制白僵菌和毛癣菌的生长,抑制区直径分别为 4-8 毫米和 7-14 毫米。内生真菌提取物--CT2 和 NL1--表现出最佳的抗菌活性,对大多数受测微生物都有抑制作用。内生真菌提取物的 HPLC-DAD 分析确定了一些化合物类别,如儿茶素衍生物、苯甲酸衍生物和芹菜素,这些化合物以前曾被报道具有抗菌潜力:结论:Newbouldia laevis 和 Cassia tora 叶子中的内生真菌能够产生具有抗感染潜力的次级代谢产物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Secondary metabolites of endophytic fungi from Newbouldia laevis and Cassia tora leaves: prospecting for new antimicrobial agents.

Aim: This study tries to prospect for new antimicrobial agents using some Nigerian plants Background: Antimicrobial compounds from fungi endophytes have shown great promise in mitigating the threats of resistant pathogens.

Objective: The study evaluated the in vitro antimicrobial property of secondary metabolites of endophytic fungi isolated from Newbouldia laevis and Cassia tora leaves.

Methods: Ten endophytic fungi were isolated from the two plants' leaves and later fermented on local rice for 21 days. Thereafter, their secondary metabolites were extracted using ethyl acetate. The antibacterial activity of the extracts on the test organisms were determined using agar diffusion and agar dilution methods, while the bioactive constituents were identified using High performance liquid chromatography coupled to diode array detector.

Results: Nine of the crude extracts (NL1, NL3, NL6, NL10, NL12, CT2, CT7, CT9 and CT10) of the fungi isolates inhibited at least one of the microorganisms studied with maximum and minimum Inhibition-Zone-Diameter of 14 mm and 2 mm respectively while CT1 did not inhibit any of the tested microorganisms at tested concentrations. The extracts exhibited good antifungal activity, inhibiting the growth of both C. albicans and Trichophyton tested with an InhibitionZone-Diameter ranging between 4-8 mm and 7-14 mm respectively. The endophytic fungi extracts- CT2 and NL1- exhibited the best antimicrobial activity, inhibiting most of the tested microorganisms. HPLC-DAD analysis of the endophytic fungal extracts identified some classes of compounds such as catechin derivatives, benzoic acid derivatives and apigenin, which were previously reported to have antimicrobial potentials.

Conclusion: Newbouldia laevis and Cassia tora leaves house endophytic fungi capable of yielding secondary metabolites with potential as anti-infective agents.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Recent patents on anti-infective drug discovery
Recent patents on anti-infective drug discovery Medicine-Pharmacology (medical)
CiteScore
2.40
自引率
0.00%
发文量
1
期刊介绍: Recent Patents on Anti-Infective Drug Discovery publishes review articles on recent patents in the field of anti-infective drug discovery e.g. novel bioactive compounds, analogs & targets. A selection of important and recent patents on anti-infective drug discovery is also included in the journal. The journal is essential reading for all researchers involved in anti-infective drug design and discovery.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信