血浆中LncRNA NCK1-AS1可区分口腔溃疡与早期口腔鳞状细胞癌。

IF 1.9 3区 生物学 Q2 BIOLOGY
Journal of Biological Research-Thessaloniki Pub Date : 2020-11-11 eCollection Date: 2020-12-01 DOI:10.1186/s40709-020-00126-1
Fei Le, Yangqian Ou, Ping Luo, Xiaoming Zhong
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引用次数: 9

摘要

背景:口腔鳞状细胞癌(OSCC)早期由于症状相似,如慢性溃疡和硬化性溃疡,容易被误诊为口腔溃疡(OU)。LncRNA NCK1-AS1被认为在宫颈癌中起关键作用,而其在OSCC中的作用尚不清楚。方法:所有受试者均于2016年12月至2018年12月在江西省肿瘤医院就诊。RT-qPCR检测OSCC和OU患者血浆中NCK1-AS1和miR-100的表达水平。通过ROC曲线进行诊断分析。通过细胞转染实验检测NCK1-AS1与miR-100之间潜在的相互作用。Transwell法检测细胞侵袭和迁移。结果:NCK1-AS1在早期OSCC患者中表达上调,而在OU患者中表达上调。NCK1-AS1的上调可将OSCC患者与OU患者区分开来。miR-100表达与NCK1-AS1表达呈负相关。过表达NCK1-AS1会促进OSCC细胞的侵袭和迁移。过表达miR-100不影响NCK1-AS1的表达,但抑制NCK1-AS1的作用。结论:NCK1-AS1可能通过下调miR-100促进OSCC转移。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

LncRNA NCK1-AS1 in plasma distinguishes oral ulcer from early-stage oral squamous cell carcinoma.

LncRNA NCK1-AS1 in plasma distinguishes oral ulcer from early-stage oral squamous cell carcinoma.

LncRNA NCK1-AS1 in plasma distinguishes oral ulcer from early-stage oral squamous cell carcinoma.

LncRNA NCK1-AS1 in plasma distinguishes oral ulcer from early-stage oral squamous cell carcinoma.

Background: Oral squamous cell carcinoma (OSCC) at early stages can be misdiagnosed as an oral ulcer (OU) due to similar symptoms, such as chronic and indurated ulcer. LncRNA NCK1-AS1 has been characterized as a key player in cervical cancer, while its role in OSCC is unknown.

Methods: All participants were selected at Jiangxi Province Tumor Hospital from December 2016 to December 2018. Expression levels of NCK1-AS1 and miR-100 in plasma from both OSCC and OU patients were measured by RT-qPCR. Diagnostic analysis was performed through ROC curve. Potential interactions between NCK1-AS1 and miR-100 were detected by cell transfection experiments. Cell invasion and migration were assessed by Transwell assays.

Results: The expression of NCK1-AS1 was upregulated in early-stage OSCC patients but not in OU patients. Upregulation of NCK1-AS1 distinguished OSCC patients from OU patients. The expression of miR-100 was inversely correlated with the expression of NCK1-AS1. Overexpression of NCK1-AS1 was followed by promoted OSCC cell invasion and migration. Overexpression of miR-100 did not affect the expression of NCK1-AS1 but inhibited the role of NCK1-AS1.

Conclusions: Therefore, NCK1-AS1 may promote the metastasis of OSCC by downregulating miR-100.

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来源期刊
CiteScore
5.20
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Journal of Biological Research-Thessaloniki is a peer-reviewed, open access, international journal that publishes articles providing novel insights into the major fields of biology. Topics covered in Journal of Biological Research-Thessaloniki include, but are not limited to: molecular biology, cytology, genetics, evolutionary biology, morphology, development and differentiation, taxonomy, bioinformatics, physiology, marine biology, behaviour, ecology and conservation.
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