Ashutosh Chaturvedi, Gopal Nath, Virender Bhadur Yadav, Meera Antiwal, Niharika Shakya, C Swathi, Jai Prakash Singh
{"title":"对肠道菌群进行 Virechana Karma(治疗性净化)的临床研究,特别是肥胖症。","authors":"Ashutosh Chaturvedi, Gopal Nath, Virender Bhadur Yadav, Meera Antiwal, Niharika Shakya, C Swathi, Jai Prakash Singh","doi":"10.4103/ayu.AYU_302_19","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Altered gut flora is associated with the pathogenesis of both intestinal and extra‑intestinal disorders. Aetiology of obesity is associated with mechanisms such as short chain fatty acid production, stimulation of hormones, chronic low‑grade inflammation, lipoprotein and bile acid metabolism and increased endocannabinoid. Receptor system tone have been suggested to explain the role of gut microbiota of obesity. The <i>Panchakarma</i> (Ayurvedic purification methods) claims the management of metabolic disorders hence this work provides the target specific evidence for the clinical studies. The proposed project is aimed to explore the particular molecular mechanism and, to make this therapy more evidence based. Hence, it was hypothesized that <i>Panchakarma</i>‑based intervention such as <i>Virechana Karma</i> (therapeutic purgation) may influence microbiota and help in the management of the obesity.</p><p><strong>Materials and methods: </strong>The study was conducted to explore the effect of <i>Virechana</i> <i>Karma</i> over the gut flora; therefore, total of 19 patients with <i>Madhyama</i> <i>Koshtha</i> diagnosed with obesity were included and received the intervention. Before and after <i>Virechana</i>, a stool sample was collected and processed for the enterobacterial repetitive intergenic consensus ‑polymerase chain reaction to find the changes over the facultative aerobic bacteria.</p><p><strong>Results: </strong>It was found that <i>Virechana</i> is effective in the management of the obesity as it helps to reduce colonization of aerobic bacteria. After <i>Virechana</i> and after follow‑up also, it showed the correction of the gut flora dysbiosis, thus initiated the weight loss mechanism in the body, resulting in diminution in the signs and symptoms of obesity.</p><p><strong>Conclusion: </strong><i>Virechana</i> is effective in the management of the obesity due to reduction in the <i>Escherichia coli</i> colonization and is effective over the gut flora dysbiosis.</p>","PeriodicalId":8720,"journal":{"name":"Ayu","volume":"40 3","pages":"179-184"},"PeriodicalIF":0.0000,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/db/dd/AYU-40-179.PMC7685265.pdf","citationCount":"0","resultStr":"{\"title\":\"A clinical study on <i>Virechana Karma</i> (therapeutic purgation) over the gut flora with special reference to obesity.\",\"authors\":\"Ashutosh Chaturvedi, Gopal Nath, Virender Bhadur Yadav, Meera Antiwal, Niharika Shakya, C Swathi, Jai Prakash Singh\",\"doi\":\"10.4103/ayu.AYU_302_19\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Altered gut flora is associated with the pathogenesis of both intestinal and extra‑intestinal disorders. Aetiology of obesity is associated with mechanisms such as short chain fatty acid production, stimulation of hormones, chronic low‑grade inflammation, lipoprotein and bile acid metabolism and increased endocannabinoid. Receptor system tone have been suggested to explain the role of gut microbiota of obesity. The <i>Panchakarma</i> (Ayurvedic purification methods) claims the management of metabolic disorders hence this work provides the target specific evidence for the clinical studies. The proposed project is aimed to explore the particular molecular mechanism and, to make this therapy more evidence based. Hence, it was hypothesized that <i>Panchakarma</i>‑based intervention such as <i>Virechana Karma</i> (therapeutic purgation) may influence microbiota and help in the management of the obesity.</p><p><strong>Materials and methods: </strong>The study was conducted to explore the effect of <i>Virechana</i> <i>Karma</i> over the gut flora; therefore, total of 19 patients with <i>Madhyama</i> <i>Koshtha</i> diagnosed with obesity were included and received the intervention. Before and after <i>Virechana</i>, a stool sample was collected and processed for the enterobacterial repetitive intergenic consensus ‑polymerase chain reaction to find the changes over the facultative aerobic bacteria.</p><p><strong>Results: </strong>It was found that <i>Virechana</i> is effective in the management of the obesity as it helps to reduce colonization of aerobic bacteria. After <i>Virechana</i> and after follow‑up also, it showed the correction of the gut flora dysbiosis, thus initiated the weight loss mechanism in the body, resulting in diminution in the signs and symptoms of obesity.</p><p><strong>Conclusion: </strong><i>Virechana</i> is effective in the management of the obesity due to reduction in the <i>Escherichia coli</i> colonization and is effective over the gut flora dysbiosis.</p>\",\"PeriodicalId\":8720,\"journal\":{\"name\":\"Ayu\",\"volume\":\"40 3\",\"pages\":\"179-184\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/db/dd/AYU-40-179.PMC7685265.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ayu\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/ayu.AYU_302_19\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/8/8 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ayu","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/ayu.AYU_302_19","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/8/8 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
A clinical study on Virechana Karma (therapeutic purgation) over the gut flora with special reference to obesity.
Background: Altered gut flora is associated with the pathogenesis of both intestinal and extra‑intestinal disorders. Aetiology of obesity is associated with mechanisms such as short chain fatty acid production, stimulation of hormones, chronic low‑grade inflammation, lipoprotein and bile acid metabolism and increased endocannabinoid. Receptor system tone have been suggested to explain the role of gut microbiota of obesity. The Panchakarma (Ayurvedic purification methods) claims the management of metabolic disorders hence this work provides the target specific evidence for the clinical studies. The proposed project is aimed to explore the particular molecular mechanism and, to make this therapy more evidence based. Hence, it was hypothesized that Panchakarma‑based intervention such as Virechana Karma (therapeutic purgation) may influence microbiota and help in the management of the obesity.
Materials and methods: The study was conducted to explore the effect of VirechanaKarma over the gut flora; therefore, total of 19 patients with MadhyamaKoshtha diagnosed with obesity were included and received the intervention. Before and after Virechana, a stool sample was collected and processed for the enterobacterial repetitive intergenic consensus ‑polymerase chain reaction to find the changes over the facultative aerobic bacteria.
Results: It was found that Virechana is effective in the management of the obesity as it helps to reduce colonization of aerobic bacteria. After Virechana and after follow‑up also, it showed the correction of the gut flora dysbiosis, thus initiated the weight loss mechanism in the body, resulting in diminution in the signs and symptoms of obesity.
Conclusion: Virechana is effective in the management of the obesity due to reduction in the Escherichia coli colonization and is effective over the gut flora dysbiosis.