{"title":"免疫功能低下感染小鼠血清中环状毛霉抗原的鉴定","authors":"Maialen Areitio , Adela Martin-Vicente , Aitana Arbizu , Aitziber Antoran , Leire Aparicio-Fernandez , Idoia Buldain , Leire Martin-Souto , Aitor Rementeria , Javier Capilla , Fernando L. Hernando , Andoni Ramirez-Garcia","doi":"10.1016/j.riam.2020.07.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><span><em>Mucor circinelloides</em></span> is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment.</p></div><div><h3>Aims</h3><p>In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of <em>M. circinelloides</em> using sera from immunocompromised infected mice.</p></div><div><h3>Methods</h3><p><span>The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of </span><em>M. circinelloides</em>.</p></div><div><h3>Results</h3><p>Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract.</p></div><div><h3>Conclusions</h3><p>In this work the most immunoreactive antigens of the secretome and the total extract of <em>M. circinelloides</em> were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.riam.2020.07.002","citationCount":"4","resultStr":"{\"title\":\"Identification of Mucor circinelloides antigens recognized by sera from immunocompromised infected mice\",\"authors\":\"Maialen Areitio , Adela Martin-Vicente , Aitana Arbizu , Aitziber Antoran , Leire Aparicio-Fernandez , Idoia Buldain , Leire Martin-Souto , Aitor Rementeria , Javier Capilla , Fernando L. Hernando , Andoni Ramirez-Garcia\",\"doi\":\"10.1016/j.riam.2020.07.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p><span><em>Mucor circinelloides</em></span> is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment.</p></div><div><h3>Aims</h3><p>In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of <em>M. circinelloides</em> using sera from immunocompromised infected mice.</p></div><div><h3>Methods</h3><p><span>The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of </span><em>M. circinelloides</em>.</p></div><div><h3>Results</h3><p>Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract.</p></div><div><h3>Conclusions</h3><p>In this work the most immunoreactive antigens of the secretome and the total extract of <em>M. circinelloides</em> were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.</p></div>\",\"PeriodicalId\":21291,\"journal\":{\"name\":\"Revista Iberoamericana De Micologia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2020-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.riam.2020.07.002\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Iberoamericana De Micologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1130140620300322\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Iberoamericana De Micologia","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1130140620300322","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
Identification of Mucor circinelloides antigens recognized by sera from immunocompromised infected mice
Background
Mucor circinelloides is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment.
Aims
In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of M. circinelloides using sera from immunocompromised infected mice.
Methods
The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of M. circinelloides.
Results
Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract.
Conclusions
In this work the most immunoreactive antigens of the secretome and the total extract of M. circinelloides were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.
期刊介绍：
Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.
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